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VISTA Polyclonal antibody

VISTA Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24849-1-AP

Synonyms

B7-H5, C10orf54, GI24, Platelet receptor Gi24, PP2135, SISP1, UNQ730/PRO1412, VISTA, VSIR

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VISTA Polyclonal antibody (24849-1-AP)

    WB analysis using 24849-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24849-1-AP (VISTA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using VISTA Polyclonal antibody (24849-1-AP)

    WB analysis of SH-SY5Y using 24849-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24849-1-AP (VISTA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using VISTA Polyclonal antibody (24849-1-AP)

    IHC staining of human tonsillitis using 24849-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24849-1-AP (VISTA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using VISTA Polyclonal antibody (24849-1-AP)

    IHC staining of human tonsillitis using 24849-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24849-1-AP (VISTA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using VISTA Polyclonal antibody (24849-1-AP)

    IHC staining of human placenta using 24849-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24849-1-AP (VISTA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using VISTA Polyclonal antibody (24849-1-AP)

    IHC staining of human placenta using 24849-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24849-1-AP (VISTA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using VISTA Polyclonal antibody (24849-1-AP)

    IHC staining of human colon cancer using 24849-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24849-1-AP (VISTA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using VISTA Polyclonal antibody (24849-1-AP)

    IHC staining of human colon cancer using 24849-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24849-1-AP (VISTA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using VISTA Polyclonal antibody (24849-1-AP)

    IF Staining of human placenta using 24849-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using VISTA antibody (24849-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using VISTA Polyclonal antibody (24849-1-AP)

    IF Staining of human placenta using 24849-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using VISTA antibody (24849-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using VISTA Polyclonal antibody (24849-1-AP)

    IF Staining of human tonsillitis using 24849-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 24849-1-AP (VISTA rabbit polyclonal antibody) at dilution of 1:100 and 60181-1-Ig (CD3 mouse monoclonal antibody), further stained with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 24849-1-AP and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 60181-1-Ig.

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using VISTA Polyclonal antibody (24849-1-AP)

    IF Staining of human placenta using 24849-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using VISTA antibody (24849-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman placenta tissue, SH-SY5Y cells, human peripheral blood platelets
Positive IHC detected inhuman placenta tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24849-1-AP targets VISTA in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VISTA fusion protein Ag20600
Full Name chromosome 10 open reading frame 54
Calculated Molecular Weight 311 aa, 34 kDa
Observed Molecular Weight45-60 kDa
GenBank Accession NumberBC020568
Gene Symbol C10orf54
Gene ID (NCBI) 64115
RRIDAB_2879755
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H and SISP1, is a member of the immunoglobulin superfamily. It serves as not only as a positive regulator but also as a substrate of MT1-MMP, and contributes at least in part to tumor invasion.

Protocols

Product Specific Protocols
WB protocol for VISTA antibody 24849-1-APDownload protocol
IHC protocol for VISTA antibody 24849-1-APDownload protocol
IF protocol for VISTA antibody 24849-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Nutr Biochem

Isothiocyanates attenuate immune checkpoint blockage therapy in gastric cancer via induction of PD-L1 expression

Authors - Qi Zhang
View Article
humanIF

Front Cell Dev Biol

Identify the Prognostic and Immune Profile of VSIR in the Tumor Microenvironment: A Pan-Cancer Analysis.

Authors - Yuanyuan Liu
View Article