FBXO41 Polyclonal antibody

FBXO41 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24519-1-AP

Synonyms

F box only protein 41, F box protein 41, Fbx41, FBXO41, KIAA1940



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 24519-1-AP is FBXO41 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXO41 fusion protein Ag21595
Full Name F-box protein 41
Calculated Molecular Weight 875 aa, 94 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC112202
Gene Symbol FBXO41
Gene ID (NCBI) 150726
RRIDAB_2879586
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBXO41 also named as F box only protein 41 is a 875 amino-acid protein, which directly interact with SKP1 and CUL1. FBXO41 is identified as a Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex, which as a regulator mediates some protein ubiquitination degradation. FBXO41 consists of F-box domain and has zinc ion binding activity. FBXO41 may have an association with Parkinson's disease.

Protocols

Product Specific Protocols
WB protocol for FBXO41 antibody 24519-1-APDownload protocol
IHC protocol for FBXO41 antibody 24519-1-APDownload protocol
IP protocol for FBXO41 antibody 24519-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Sci Adv

Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.

Authors - Odetta Antico
ratWB

iScience

Neuronal F-Box protein FBXO41 regulates synaptic transmission and hippocampal network maturation.

Authors - Ana R A A Quadros