Validation Data Gallery
Tested Applications
| Positive WB detected in | human placenta tissue, mouse ovary tissue, mouse uterus tissue |
| Positive IHC detected in | mouse ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22324-1-AP targets OVGP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17771 Product name: Recombinant human OVGP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 329-678 aa of BC126177 Sequence: VGYDNAISFSYKAWFIRREHFGGAMVWTLDMDDVRGTFCGTGPFPLVYVLNDILVRAEFSSTSLPQFWLSSAVNSSSTDPERLAVTTAWTTDSKILPPGGEAGVTEIHGKCENMTITPRGTTVTPTKETVSLGKHTVALGEKTEITGAMTMTSVGHQSMTPGEKALTPVGHQSVTTGQKTLTSVGYQSVTPGEKTLTPVGHQSVTPVSHQSVSPGGTTMTPVHFQTETLRQNTVAPRRKAVAREKVTVPSRNISVTPEGQTMPLRGENLTSEVGTHPRMGNLGLQMEAENRMMLSSSPVIQLPEQTPLAFDNRFVPIYGNHSSVNSVTPQTSPLSLKKEIPENSAVDEEA 相同性解析による交差性が予測される生物種 |
| Full Name | oviductal glycoprotein 1, 120kDa |
| Calculated molecular weight | 678 aa, 75 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC126177 |
| Gene Symbol | OVGP1 |
| Gene ID (NCBI) | 5016 |
| RRID | AB_3085696 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q12889 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
OVGP1 (oviduct-specific glycoprotein, also known as MUC9) is a high molecular-weight glycoprotein thought to be exclusively secreted by the non-ciliated epithelial cells of the fallopian tube (PMID: 12444068). Identified first as an estrogen-induced secretory protein in the oviduct, OVGP1 aids in sperm capacitation, fertilization, and early embryonic development (PMID: 28883914). OVGP1 is the major non-serum protein present in OF (oviduct fluid) and its biological activity differs among species (PMID: 27601270).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for OVGP1 antibody 22324-1-AP | Download protocol |
| WB protocol for OVGP1 antibody 22324-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |