Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of LNCaP using 22124-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22124-1-AP (TBC1D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293T using 22124-1-AP
WB result of TBC1D1 antibody (22124-1-AP; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-TBC1D1 transfected HEK-293T cells.
WB analysis using 22124-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22124-1-AP (TBC1D1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 22124-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 22124-1-AP (TBC1D1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 22124-1-AP
IP result of anti-TBC1D1 (IP:22124-1-AP, 4ug; Detection:22124-1-AP 1:1000) with Jurkat cells lysate 2400ug.
IHC staining of human testis using 22124-1-AP
Immunohistochemical analysis of paraffin-embedded human testis slide using 22124-1-AP (TBC1D1 Antibody) at dilution of 1:50.
IHC staining of human testis using 22124-1-AP
Immunohistochemical analysis of paraffin-embedded human testis slide using 22124-1-AP (TBC1D1 Antibody) at dilution of 1:50.
IHC staining of mouse kidney using 22124-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22124-1-AP (TBC1D1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 22124-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22124-1-AP (TBC1D1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 22124-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney slide using 22124-1-AP (TBC1D1 Antibody) at dilution of 1:50.
IHC staining of human kidney using 22124-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney slide using 22124-1-AP (TBC1D1 Antibody) at dilution of 1:50.

Featured Product
KD/KO Validated

TBC1D1 Polyclonal antibody

TBC1D1 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 22124-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Synonyms

KIAA1108, TBC, TBC1, TBC1 domain family member 1, TBC1D1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (12)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	LNCaP cells, HeLa cells, HEK-293T cells, Jurkat cells, MCF-7 cells
Positive IP detected in	Jurkat cells
Positive IHC detected in	mouse kidney tissue, human testis tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 11 publications below
IHC	See 2 publications below
IP	See 1 publications below

Product Information

22124-1-AP targets TBC1D1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5406 Product name: Recombinant human TBC1D1 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 813-1168 aa of BC050321 Sequence: LAEQFHLKHQFPSKQQPKDVPYKELLKQLTSQQHAILIDLGRTFPTHPYFSAQLGAGQLSLYNILKAYSLLDQEVGYCQGLSFVAGILLLHMSEEEAFKMLKFLMFDMGLRKQYRPDMIILQIQMYQLSRLLHDYHRDLYNHLEEHEIGPSLYAAPWFLTMFASQFPLGFVARVFDMIFLQGTEVIFKVALSLLGSHKPLILQHENLETIVDFIKSTLPNLGLVQMEKTINQVFEMDIAKQLQAYEVEYHVLQEELIDSSPLSDNQRMDKLEKTNSSLRKQNLDLLEQLQVANGRIQSLEATIEKLLSSESKLKQAMLTLELERSALLQTVEELRRRSAEPSDREPECTQPEPTGD 相同性解析による交差性が予測される生物種
Full Name	TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1
Calculated molecular weight	133 kDa
Observed molecular weight	150-165 kDa
GenBank accession number	BC050321
Gene Symbol	TBC1D1
Gene ID (NCBI)	23216
RRID	AB_2827874
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q86TI0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for TBC1D1 antibody 22124-1-AP	Download protocol
IP protocol for TBC1D1 antibody 22124-1-AP	Download protocol
WB protocol for TBC1D1 antibody 22124-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Metab Skeletal muscle from TBC1D4 p.Arg684Ter variant carriers is severely insulin resistant but exhibits normal metabolic responses during exercise Authors - Jonas M Kristensen View Article
human	WB,IP	Diabetes Illumination of the Endogenous Insulin-Regulated TBC1D4 Interactome in Human Skeletal Muscle. Authors - Jeppe K Larsen View Article
mouse	WB	Mol Nutr Food Res Quercetin oppositely regulates insulin-mediated glucose disposal in skeletal muscle under normal and inflammatory conditions: The dual roles of AMPK activation. Authors - Kang Liu View Article
human	WB	J Physiol The insulin-sensitizing effect of a single exercise bout is similar in type I and type II human muscle fibres. Authors - Magnus R Larsen View Article
		J Biol Chem Cooperative actions of Tbc1d1 and AS160/Tbc1d4 in GLUT4 trafficking activities. Authors - Hiroyasu Hatakeyama View Article
mouse	WB	J Pharmacol Sci PMID: 30733181 Authors - Zejun Mo View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of LNCaP using 22124-1-AP

WB analysis of HEK-293T using 22124-1-AP

WB analysis using 22124-1-AP

WB analysis of HeLa using 22124-1-AP

IP experiment of Jurkat using 22124-1-AP

IHC staining of human testis using 22124-1-AP

IHC staining of human testis using 22124-1-AP

IHC staining of mouse kidney using 22124-1-AP

IHC staining of mouse kidney using 22124-1-AP

IHC staining of human kidney using 22124-1-AP

IHC staining of human kidney using 22124-1-AP

TBC1D1 Polyclonal antibody

TBC1D1 Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Skeletal muscle from TBC1D4 p.Arg684Ter variant carriers is severely insulin resistant but exhibits normal metabolic responses during exercise

Illumination of the Endogenous Insulin-Regulated TBC1D4 Interactome in Human Skeletal Muscle.

Quercetin oppositely regulates insulin-mediated glucose disposal in skeletal muscle under normal and inflammatory conditions: The dual roles of AMPK activation.

The insulin-sensitizing effect of a single exercise bout is similar in type I and type II human muscle fibres.

Cooperative actions of Tbc1d1 and AS160/Tbc1d4 in GLUT4 trafficking activities.

PMID: 30733181