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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using Podoplanin Polyclonal antibody (22099-1-AP)

    WB analysis of human brain using 22099-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 22099-1-AP (Podoplanin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using Podoplanin Polyclonal antibody (22099-1-AP)

    WB analysis of human brain using 22099-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 22099-1-AP (Podoplanin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using Podoplanin Polyclonal antibody (22099-1-AP)

    WB analysis of human placenta using 22099-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 22099-1-AP (Podoplanin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using Podoplanin Polyclonal antibody (22099-1-AP)

    IHC staining of human appendicitis using 22099-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 22099-1-AP (Podoplanin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using Podoplanin Polyclonal antibody (22099-1-AP)

    IHC staining of human appendicitis using 22099-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 22099-1-AP (Podoplanin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Podoplanin Polyclonal antibody (22099-1-AP)

    IHC staining of human lymphoma using 22099-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 22099-1-AP (Podoplanin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Podoplanin Polyclonal antibody (22099-1-AP)

    IHC staining of human lymphoma using 22099-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 22099-1-AP (Podoplanin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using Podoplanin Polyclonal antibody (22099-1-AP)

    IF Staining of human placenta using 22099-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Podoplanin antibody (22099-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using Podoplanin Polyclonal antibody (22099-1-AP)

    IF Staining of human placenta using 22099-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Podoplanin antibody (22099-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Podoplanin Polyclonal antibody

Podoplanin Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 22099-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Synonyms

PDPN, PSEC0003, PA2.26 antigen, Aggrus, 29kDa cytosolic podoplanin intracellular domain

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inhuman brain tissue, human placenta tissue
Positive IHC detected inhuman appendicitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22099-1-AP targets Podoplanin in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag17307

Product name: Recombinant human PDPN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 101-238 aa of BC022812

Sequence: TGQPEDDTETTGLEGGVAMPGAEDDVVTPGTSEDRYKSGLTTLVATSVNSVTGIRIEDLPTSESTVHAQEQSPSATASNVATSHSTEKVDGDTQTTVEKDGLSTVTLVGIIVGVLLAIGFIGGIIVVVMRKMSGRYSP

相同性解析による交差性が予測される生物種
Full Name podoplanin
Calculated molecular weight 238 aa, 25 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC022812
Gene Symbol Podoplanin
Gene ID (NCBI) 10630
RRIDAB_2878995
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86YL7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Podoplanin antibody 22099-1-APDownload protocol
IHC protocol for Podoplanin antibody 22099-1-APDownload protocol
IF protocol for Podoplanin antibody 22099-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols