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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using WBSCR17 Polyclonal antibody (21280-1-AP)

    WB analysis of mouse brain using 21280-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21280-1-AP (WBSCR17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using WBSCR17 Polyclonal antibody (21280-1-AP)

    WB analysis of mouse lung using 21280-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 21280-1-AP (WBSCR17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using WBSCR17 Polyclonal antibody (21280-1-AP)

    WB analysis of A549 using 21280-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 21280-1-AP (WBSCR17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using WBSCR17 Polyclonal antibody (21280-1-AP)

    WB analysis of human brain using 21280-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 21280-1-AP (WBSCR17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using WBSCR17 Polyclonal antibody (21280-1-AP)

    IHC staining of human brain using 21280-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21280-1-AP (WBSCR17 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using WBSCR17 Polyclonal antibody (21280-1-AP)

    IHC staining of human brain using 21280-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21280-1-AP (WBSCR17 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using WBSCR17 Polyclonal antibody (21280-1-AP)

    IHC staining of mouse brain using 21280-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21280-1-AP (WBSCR17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using WBSCR17 Polyclonal antibody (21280-1-AP)

    IHC staining of human heart using 21280-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21280-1-AP (WBSCR17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using WBSCR17 Polyclonal antibody (21280-1-AP)

    IHC staining of human prostate cancer using 21280-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21280-1-AP (WBSCR17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using WBSCR17 Polyclonal antibody (21280-1-AP)

    IHC staining of mouse cerebellum using 21280-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 21280-1-AP (WBSCR17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using WBSCR17 Polyclonal antibody (21280-1-AP)

    IHC staining of human heart using 21280-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 21280-1-AP (WBSCR17 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using WBSCR17 Polyclonal antibody (21280-1-AP)

    IHC staining of human heart using 21280-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 21280-1-AP (WBSCR17 antibody) at dilution of 1:100 (under 40x lens).

WBSCR17 Polyclonal antibody

WBSCR17 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21280-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

EC:2.4.1.41, GalNAc T like protein 3, GalNAc-T-like protein 3, GALNT17, Polypeptide GalNAc transferase-like protein 3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, A549 cells, mouse lung tissue, human brain tissue
Positive IHC detected inhuman brain tissue, mouse brain tissue, mouse cerebellum tissue, human heart tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21280-1-AP targets WBSCR17 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15792

Product name: Recombinant human WBSCR17 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-126 aa of BC069624

Sequence: MASLRRVKVLLVLNLIAVAGFVLFLAKCRPIAVRSGDAFHEIRPRAEVANLSAHSASPIQDAVLKRLSLLEDIVYRQLNGLSKSLGLIEGYGGRGKGGLPATLSPAEEEKAKGPHEKYGYNSYLSE

相同性解析による交差性が予測される生物種
Full Name Williams-Beuren syndrome chromosome region 17
Calculated molecular weight 598 aa, 68 kDa
Observed molecular weight70~90 kDa
GenBank accession numberBC069624
Gene Symbol WBSCR17
Gene ID (NCBI) 64409
RRIDAB_10732815
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6IS24
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WBSCR17, also known as GALNT17, which encodes a brain-expressed N-acetylgalactosaminyl transferase (GalNAcT), is located at the distal edge of a region that is commonly deleted or duplicated in Williams Beuren Syndrome (WBS), a developmental disorder with motor and coordination problems, impaired visuospatial memory, and abnormal social interaction (PMID: 31554716). WBSCR17 loss-of-function has significant effects on cerebellar development, and is associated with phenotypes including developmental delay, deficits in motor coordination, reduced exploratory activity, and impaired social behavior (PMID: 22787146). With the calculated molecular mass of recombinant WBSCR17 being 68 kDa, the 70-90-kDa glycoproteins could also be detected due to post-translational modifications (PMID: 22787146).

Protocols

Product Specific Protocols
IHC protocol for WBSCR17 antibody 21280-1-APDownload protocol
WB protocol for WBSCR17 antibody 21280-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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