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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using KIAA0528 Polyclonal antibody (21138-1-AP)

    WB analysis of HeLa using 21138-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using KIAA0528 Polyclonal antibody (21138-1-AP)

    WB analysis using 21138-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using KIAA0528 Polyclonal antibody (21138-1-AP)

    WB analysis of HeLa using 21138-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21138-1-AP (KIAA0528 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using KIAA0528 Polyclonal antibody (21138-1-AP)

    IP experiment of HeLa using 21138-1-AP

    IP result of anti-KIAA0528 (IP:21138-1-AP, 4ug; Detection:21138-1-AP 1:700) with HeLa cells lysate 520ug.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using KIAA0528 Polyclonal antibody (21138-1-AP)

    IHC staining of mouse small intestine using 21138-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using KIAA0528 Polyclonal antibody (21138-1-AP)

    IHC staining of mouse small intestine using 21138-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using KIAA0528 Polyclonal antibody (21138-1-AP)

    IHC staining of mouse lung using 21138-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using KIAA0528 Polyclonal antibody (21138-1-AP)

    IHC staining of mouse ovary using 21138-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using KIAA0528 Polyclonal antibody (21138-1-AP)

    IHC staining of mouse ovary using 21138-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using KIAA0528 Polyclonal antibody (21138-1-AP)

    IHC staining of human ovary cancer using 21138-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using KIAA0528 Polyclonal antibody (21138-1-AP)

    IHC staining of mouse ovary using 21138-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using KIAA0528 Polyclonal antibody (21138-1-AP)

    IHC staining of mouse ovary using 21138-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KIAA0528 Polyclonal antibody (21138-1-AP)

    IF Staining of HepG2 using 21138-1-AP

    Immunofluorescent analysis of HepG2 cells, using KIAA0528 antibody 21138-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

KIAA0528 Polyclonal antibody

KIAA0528 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 21138-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

C2 domain-containing phosphoprotein of 138 kDa, C2 domain-containing protein 5, C2CD5, CDP138

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse small intestine tissue, mouse lung tissue, human ovary cancer tissue, mouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21138-1-AP targets KIAA0528 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15590

Product name: Recombinant human KIAA0528 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 651-1000 aa of BC042498

Sequence: RSRLLRSQSESSDEVTELDLSHGKKDAFVLEIDDTDAMEDVHSLLTDVPPPSGFYSCNTEIMPGINNWTSEIQMFTSVRVIRLSSLNLTNQALNKNFNDLCENLLKSLYFKLRSMIPCCLCHVNFTVSLPEDELIQVTVTAVAITFDKNQALQTTKTPVEKSLQRASTDNEELLQFPLELCSDSLPSHPFPPAKAMTVEKASPVGDGNFRNRSAPPCANSTVGVVKMTPLSFIPGAKITKYLGIINMFFIRETTSLREEGGVSGFLHAFIAEVFAMVRAHVAALGGNAVVSYIMKQCVFMENPNKNQAQCLINVSGDAVVFVRESDLEVVSSQQPTTNCQSSCTEGEVTT

相同性解析による交差性が予測される生物種
Full Name KIAA0528
Calculated molecular weight 1000 aa, 110 kDa
Observed molecular weight 110-125 kDa
GenBank accession numberBC042498
Gene Symbol KIAA0528
Gene ID (NCBI) 9847
RRIDAB_10858480
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86YS7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for KIAA0528 antibody 21138-1-APDownload protocol
IHC protocol for KIAA0528 antibody 21138-1-APDownload protocol
IP protocol for KIAA0528 antibody 21138-1-APDownload protocol
WB protocol for KIAA0528 antibody 21138-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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