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Validation Data Gallery

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  • Western Blot (WB) analysis of ROS1728 cells using C10orf58 Polyclonal antibody (20446-1-AP)

    WB analysis of ROS1728 using 20446-1-AP

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 20446-1-AP (C10orf58 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin tissue using C10orf58 Polyclonal antibody (20446-1-AP)

    IHC staining of human skin using 20446-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 20446-1-AP (C10orf58 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using C10orf58 Polyclonal antibody (20446-1-AP)

    IHC staining of human skin using 20446-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 20446-1-AP (C10orf58 antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of ROS1728 cells using C10orf58 Polyclonal antibody (20446-1-AP)

    IF Staining of ROS1728 using 20446-1-AP

    Immunofluorescent analysis of ROS1728 cells using 20446-1-AP (C10orf58 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of ROS1728 cells using C10orf58 Polyclonal antibody (20446-1-AP)

    IF Staining of ROS1728 using 20446-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed ROS1728 cells using C10orf58 antibody (20446-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

C10orf58 Polyclonal antibody

C10orf58 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20446-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

F213A, FAM213A, PAMM, Peroxiredoxin-like 2 activated in M-CSF stimulated monocytes, Peroxiredoxin-like 2A

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inROS1728 cells
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inROS1728 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20446-1-AP targets C10orf58 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14272

Product name: Recombinant human C10orf58 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 35-229 aa of BC005871

Sequence: DVFLSKPQKAALEYLEDIDLKTLEKEPRTFKAKELWEKNGAVIMAVRRPGCFLCREEAADLSSLKSMLDQLGVPLYAVVKEHIRTEVKDFQPYFKGEIFLDEKKKFYGPQRRKMMFMGFIRLGVWYNFFRAWNGGFSGNLEGEGFILGGVFVVGSGKQGILLEHREKEFGDKVNLLSVLEAAKMIKPQTLASEKK

相同性解析による交差性が予測される生物種
Full Name chromosome 10 open reading frame 58
Calculated molecular weight 229 aa, 26 kDa
Observed molecular weight 24-30 kDa
GenBank accession numberBC005871
Gene Symbol C10orf58
Gene ID (NCBI) 84293
RRIDAB_2878687
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRX8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C10orf58, also name as FAM213A and PAMM, is involved in redox regulation of the cell. It acts as an antioxidant. C10orf58 inhibits TNFSF11-induced NFKB1 and JUN activation and osteoclast differentiation. It may affect bone resorption and help to maintain bone mass. The MW of C10orf58 is 24-30 kDa.

Protocols

Product Specific Protocols
IF protocol for C10orf58 antibody 20446-1-APDownload protocol
IHC protocol for C10orf58 antibody 20446-1-APDownload protocol
WB protocol for C10orf58 antibody 20446-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Rep

The miR4261/PAMM axis in multiple myeloma promotes bone resorption.

Authors - Jiangxia Cui
  • KD Validated
View Article
HumanIHC

Oncol Rep

Overexpression of ZNF169 promotes the growth and proliferation of colorectal cancer cells via the upregulation of ANKZF1

Authors - Jie Zhang
View Article
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