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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MIS12 Polyclonal antibody (19820-1-AP)

    WB analysis using 19820-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19820-1-AP (MIS12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using MIS12 Polyclonal antibody (19820-1-AP)

    IHC staining of mouse kidney using 19820-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using MIS12 Polyclonal antibody (19820-1-AP)

    IHC staining of mouse ovary using 19820-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat ovary tissue using MIS12 Polyclonal antibody (19820-1-AP)

    IHC staining of rat ovary using 19820-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using MIS12 Polyclonal antibody (19820-1-AP)

    IHC staining of rat small intestine using 19820-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using MIS12 Polyclonal antibody (19820-1-AP)

    IHC staining of human ovary cancer using 19820-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using MIS12 Polyclonal antibody (19820-1-AP)

    IHC staining of human placenta using 19820-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using MIS12 Polyclonal antibody (19820-1-AP)

    IHC staining of mouse ovary using 19820-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using MIS12 Polyclonal antibody (19820-1-AP)

    IHC staining of mouse small intestine using 19820-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using MIS12 Polyclonal antibody (19820-1-AP)

    IHC staining of rat kidney using 19820-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat ovary tissue using MIS12 Polyclonal antibody (19820-1-AP)

    IHC staining of rat ovary using 19820-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using MIS12 Polyclonal antibody (19820-1-AP)

    IHC staining of rat small intestine using 19820-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using MIS12 Polyclonal antibody (19820-1-AP)

    IHC staining of human ovary cancer using 19820-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using MIS12 Polyclonal antibody (19820-1-AP)

    IHC staining of human ovary cancer using 19820-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MIS12 Polyclonal antibody (19820-1-AP)

    IF Staining of HeLa using 19820-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MIS12 antibody (19820-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

MIS12 Polyclonal antibody

MIS12 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19820-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

hMis12, KNTC2AP, MTW1, Protein MIS12 homolog

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman ovary cancer tissue, mouse ovary tissue, rat small intestine tissue, human placenta tissue, mouse small intestine tissue, rat kidney tissue, rat ovary tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19820-1-AP targets MIS12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13870

Product name: Recombinant human MIS12 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-205 aa of BC000229

Sequence: MSVDPMTYEAQFFGFTPQTCMLRIYIAFQDYLFEVMQAVEQVILKKLDGIPDCDISPVQIRKCTEKFLCFMKGHFDNLFSKMEQLFLQLILRIPSNILLPEDKCKETPYSEEDFQHLQKEIEQLQEKYKTELCTKQALLAELEEQKIVQAKLKQTLTFFDELHNVGRDHGTSDFRESLVSLVQNSRKLQNIRDNVEKESKRLKIS

相同性解析による交差性が予測される生物種
Full Name MIS12, MIND kinetochore complex component, homolog (yeast)
Calculated molecular weight 205 aa, 24 kDa
Observed molecular weight 25-28 kDa
GenBank accession numberBC000229
Gene Symbol MIS12
Gene ID (NCBI) 79003
RRIDAB_3669315
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H081
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The MIS12 complex, composed of the Mis12, Pmf1, Nsl1 and Dsn1 subunits, is a highly conserved kinetochore protein that interacts with the Ndc80 and Knl1 complexes, forming the KMN network (PMID: 32341029). MIS12 is a part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis. MIS12 is essential for proper kinetochore microtubule attachments (PubMed:23891108)

Protocols

Product Specific Protocols
IF protocol for MIS12 antibody 19820-1-APDownload protocol
IHC protocol for MIS12 antibody 19820-1-APDownload protocol
WB protocol for MIS12 antibody 19820-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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