NEB-Specific Polyclonal antibody

NEB-Specific Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19706-1-AP

Synonyms

NEB, NEB177D, nebulin, NEM2



Tested Applications

Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

19706-1-AP targets NEB-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name nebulin
Calculated Molecular Weight 773 kDa
GenBank Accession NumberNM_004543
Gene Symbol NEB
Gene ID (NCBI) 4703
RRIDAB_10637999
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NEB, a giant muscle protein, may be involved in maintaining the structural integrity of sarcomeres and the membrane system associated with the myofibrils. NEB binds and stabilizes F-actin. Defects in NEB are the cause of nemaline myopathy type 2 (NEM2). The antibody is specific to NEB.

Protocols

Product Specific Protocols
IHC protocol for NEB-Specific antibody 19706-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Clin Invest

KLHL40 deficiency destabilizes thin filament proteins and promotes nemaline myopathy.

Authors - Ankit Garg
mouseWB

Elife

KLHL41 stabilizes skeletal muscle sarcomeres by nonproteolytic ubiquitination.

Authors - Andres Ramirez-Martinez