Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of MCF-7 using 19649-1-AP
WB result of Histone H1.2 antibody (19649-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Histone H1.2 transfected MCF-7 cells.
WB analysis of L02 using 19649-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 19649-1-AP (HIST1H1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 19649-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 19649-1-AP (HIST1H1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 19649-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 19649-1-AP (HIST1H1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 19649-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 19649-1-AP (HIST1H1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 19649-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 19649-1-AP (HIST1H1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 19649-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 19649-1-AP (HIST1H1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 19649-1-AP
IP result of anti-Histone H1.2 (IP:19649-1-AP, 3ug; Detection:19649-1-AP 1:500) with HeLa cells lysate 1600ug.
IHC staining of human ovary tumor using 19649-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 19649-1-AP (HIST1H1C antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human normal colon using 19649-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 19649-1-AP (Histone H1.2 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 19649-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 19649-1-AP (HIST1H1C antibody) at dilution of 1:100 (under 10x lens).
IF Staining of HeLa using 19649-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Histone H1.2 antibody (19649-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 19649-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Histone H1.2 antibody (19649-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of HeLa using 19649-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Histone H1.2 antibody (19649-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

Featured Product
KD/KO Validated

Histone H1.2 Polyclonal antibody

Histone H1.2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 19649-1-AP

Publications(35)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Synonyms

Histone H1, histone cluster 1, H1c, HIST1H1C, H1F2, H1-2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (35)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	L02 cells, human testis tissue, Jurkat cells, MCF-7 cells, A375 cells, mouse thymus tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human ovary tumor tissue, human normal colon **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:600
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 26 publications below
IHC	See 1 publications below
IF	See 2 publications below
IP	See 2 publications below
ELISA	See 1 publications below
CoIP	See 1 publications below
ChIP	See 1 publications below

Product Information

19649-1-AP targets Histone H1.2 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, camelus bactrianus
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7503 Product name: Recombinant human Histone H1.2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-213 aa of BC002649 Sequence: MSETAPAAPAAAPPAEKAPVKKKAAKKAGGTPRKASGPPVSELITKAVAASKERSGVSLAALKKALAAAGYDVEKNNSRIKLGLKSLVSKGTLVQTKGTGASGSFKLNKKAASGEAKPKVKKAGGTKPKKPVGAAKKPKKAAGGATPKKSAKKTPKKAKKPAAATVTKKVAKSPKKAKVAKPKKAAKSAAKAVKPKAAKPKVVKPKKAAPKKK 相同性解析による交差性が予測される生物種
Full Name	histone cluster 1, H1c
Calculated molecular weight	21 kDa
Observed molecular weight	32-33 kDa
GenBank accession number	BC002649
Gene Symbol	Histone H1.2
Gene ID (NCBI)	3006
RRID	AB_10694432
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P16403
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Histone H1 regulates chromatin competency through its high affinity binding to linker DNA as a structural component. It acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation. The calculated molecular weight of Histone H1.2 is 21 kDa, but modified Histone H1.2 is about 32 kDa. (PMID: 18258596)

Protocols

Product Specific Protocols
IF protocol for Histone H1.2 antibody 19649-1-AP	Download protocol
IHC protocol for Histone H1.2 antibody 19649-1-AP	Download protocol
IP protocol for Histone H1.2 antibody 19649-1-AP	Download protocol
WB protocol for Histone H1.2 antibody 19649-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Cell Mol Immunol Mapping the holonomic signaling network that drives pathological changes in endotoxic shock. Authors - Teng Teng View Article
human	WB	Cancer Res A20/TNFAIP3 Regulates the DNA Damage Response and Mediates Tumor Cell Resistance to DNA-Damaging Therapy. Authors - Chuanzhen Yang View Article
mouse	WB	Nat Neurosci Genome-wide distribution of linker histone H1.0 is independent of MeCP2. Authors - Aya Ito-Ishida View Article
mouse	WB	Nat Commun Histone functions as a cell-surface receptor for AGEs. Authors - Masanori Itakura View Article
human	ChIP	Nat Commun The nucleic acid binding protein SFPQ represses EBV lytic reactivation by promoting histone H1 expression Authors - Laura A Murray-Nerger View Article
human	WB	Mol Cell CRAMP1 drives linker histone expression to enable Polycomb repression Authors - Rachael E Matthews View Article

Validation Data Gallery

WB analysis of MCF-7 using 19649-1-AP

WB analysis of L02 using 19649-1-AP

WB analysis of human testis using 19649-1-AP

WB analysis of mouse thymus using 19649-1-AP

WB analysis of Jurkat using 19649-1-AP

WB analysis of MCF-7 using 19649-1-AP

WB analysis of A375 using 19649-1-AP

IP experiment of HeLa using 19649-1-AP

IHC staining of human ovary tumor using 19649-1-AP

IHC staining of human normal colon using 19649-1-AP

IHC staining of human ovary tumor using 19649-1-AP

IF Staining of HeLa using 19649-1-AP

IF Staining of HepG2 using 19649-1-AP

IF Staining of HeLa using 19649-1-AP

Histone H1.2 Polyclonal antibody

Histone H1.2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Mapping the holonomic signaling network that drives pathological changes in endotoxic shock.

A20/TNFAIP3 Regulates the DNA Damage Response and Mediates Tumor Cell Resistance to DNA-Damaging Therapy.

Genome-wide distribution of linker histone H1.0 is independent of MeCP2.

Histone functions as a cell-surface receptor for AGEs.

The nucleic acid binding protein SFPQ represses EBV lytic reactivation by promoting histone H1 expression

CRAMP1 drives linker histone expression to enable Polycomb repression

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