Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells, mouse brain tissue, Y79 cells, SH-SY5Y cells, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
Product Information
18898-1-AP targets NEURL in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13531 Product name: Recombinant human NEURL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-345 aa of BC026336 Sequence: MGNNFSSIPSLPRGNPSRAPRGHPQNLKDSIGGPFPVTSHRCHHKQKHCPAVLPSGGLPAAPLLFHPHTKGSQILMDLSHKAVKRQASFCNAITFSNRPVLIYEQVRLKITKKQCCWSGALRLGFTSKDPSRIHPDSLPKYACPDLVSQSGFWAKALPEEFANEGNIIAFWVDKKGRVFHRINDSAVMLFFSGVRTADPLWALVDVYGLTRGVQLLDSELVLPDCLRPRSFTALRRPSLRREADDARLSVSLCDLNVPGADGDEAAPAASCPIPQNSLNSQHSRALPAQLDGDLRFHALRAGAHVRILDEQTVARVEHGRDERALVFTSRPVRVAETIFVKVTRS 相同性解析による交差性が予測される生物種 |
| Full Name | neuralized homolog (Drosophila) |
| Calculated molecular weight | 59 kDa |
| Observed molecular weight | 60-62 kDa |
| GenBank accession number | BC026336 |
| Gene Symbol | NEURL |
| Gene ID (NCBI) | 9148 |
| RRID | AB_10642557 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O76050 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NEURL(Neuralized-like protein 1A) is also named as NEURL1, NEURL1A, RNF67.Neurl1 is a RING-dependent E3 ubiquitin ligase that can regulate Notch signaling in mammals and it expression promotes lysosomal degradation of Jagged1 in cells and blocks signaling from Jagged1-expressing cells to neighboring Notch-expressing cells. Neurl-dependent turnover of Jagged1 and inhibition of Notch signaling depends upon N-terminal myristoylation and plasma membrane localization of Neurl1(PMID:18077452).This antibody is specific to NEURL.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NEURL antibody 18898-1-AP | Download protocol |
| IHC protocol for NEURL antibody 18898-1-AP | Download protocol |
| IP protocol for NEURL antibody 18898-1-AP | Download protocol |
| WB protocol for NEURL antibody 18898-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
