SHROOM1 Polyclonal antibody

SHROOM1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 18218-1-AP

Synonyms

Apical protein 2, APXL2, KIAA1960, Protein Shroom1, shroom family member 1, SHROOM1



Tested Applications

Positive WB detected inMCF7 cells, A375 cells
Positive IP detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 18218-1-AP is SHROOM1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHROOM1 fusion protein Ag12977
Full Name shroom family member 1
Calculated Molecular Weight 852 aa, 91 kDa
Observed Molecular Weight 100-130 kDa
GenBank Accession NumberBC104914
Gene Symbol SHROOM1
Gene ID (NCBI) 134549
RRIDAB_10858616
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SHROOM1 antibody 18218-1-APDownload protocol
IP protocol for SHROOM1 antibody 18218-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS Pathog

Defining the proteolytic landscape during enterovirus infection.

Authors - Mohsan Saeed