Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse skeletal muscle tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 6 publications below |
| IHC | See 2 publications below |
| IF | See 8 publications below |
| RIP | See 1 publications below |
Product Information
18178-1-AP targets RBM24 in WB, IHC, IF-P, RIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12871 Product name: Recombinant human RBM24 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-191 aa of BC104808 Sequence: MYVCLCVSVAKVTMADRAAAERACKDPNPIIDGRKANVNLAYLGAKPRIMQPGFAFGVQQLHPALIQRPFGIPAHYVYPQAFVQPGVVIPHVQPTAAAASTTPYIDYTGAAYAQYSAAAAAAAAAAAYDQYPYAASPAAAGYVTAGGYGYAVQQPITAAAPGTAAAAAAAAAAAAAFGQYQPQQLQTDRMQ 相同性解析による交差性が予測される生物種 |
| Full Name | RNA binding motif protein 24 |
| Calculated molecular weight | 191 aa, 20 kDa |
| Observed molecular weight | 18-25 kDa |
| GenBank accession number | BC104808 |
| Gene Symbol | RBM24 |
| Gene ID (NCBI) | 221662 |
| RRID | AB_2878513 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BX46 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RNA-binding proteins have a pivotal role in post-transcriptionally regulating gene expression. During early development, they exhibit temporally and spatially regulated expression patterns and affect the proteome of a single cell by controlling alternative splicing, transport, localization, and stability of their target RNAs (PMID: 22510371). Their expression is tissue-specific, and their crucial influence on the protein setup, especially in muscle cells, has become recognized recently (PMID: 21982546). RBM24 is one RNA-binding protein that is fundamental in regulating cardiac gene expression, sarcomeric assembly, and cardiac contractility. It may thus represent a novel pathway to cardiomyopathy (PMID: 20338237).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RBM24 antibody 18178-1-AP | Download protocol |
| IHC protocol for RBM24 antibody 18178-1-AP | Download protocol |
| WB protocol for RBM24 antibody 18178-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Differ Tropomyosin1 isoforms underlie epithelial to mesenchymal plasticity, metastatic dissemination, and resistance to chemotherapy in high-grade serous ovarian cancer | ||
Proc Natl Acad Sci U S A Alternative splicing of the Rbm24 gene is essential for cochlear hair cell stereocilia integrity and hearing function in mice
| ||
Int J Biol Macromol Rbm24 modulates neuronal RNA splicing to restrict cognitive dysfunction | ||
J Mol Med (Berl) Mechanism of METTL14-mediated m6A modification in non-small cell lung cancer cell resistance to cisplatin | ||
Sci Rep Rbm24 displays dynamic functions required for myogenic differentiation during muscle regeneration.
| ||
Development Mosaic CRISPR-stop enables rapid phenotyping of nonsense mutations in essential genes. |