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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUBP1 Polyclonal antibody (18011-1-AP)

    WB analysis using 18011-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18011-1-AP (NUBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NUBP1 Polyclonal antibody (18011-1-AP)

    WB analysis of HeLa using 18011-1-AP

    WB result of NUBP1 antibody (18011-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUBP1 transfected HeLa cells.

  • Immunoprecipitation (IP) experiment of Jurkat cells using NUBP1 Polyclonal antibody (18011-1-AP)

    IP experiment of Jurkat using 18011-1-AP

    IP result of anti-NUBP1 (IP:18011-1-AP, 4ug; Detection:18011-1-AP 1:300) with Jurkat cells lysate 3600ug.

  • Immunohistochemistry (IHC) staining of human stomach tissue using NUBP1 Polyclonal antibody (18011-1-AP)

    IHC staining of human stomach using 18011-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 18011-1-AP (NUBP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using NUBP1 Polyclonal antibody (18011-1-AP)

    IHC staining of mouse kidney using 18011-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18011-1-AP (NUBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NUBP1 Polyclonal antibody (18011-1-AP)

    IHC staining of human kidney using 18011-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18011-1-AP (NUBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using NUBP1 Polyclonal antibody (18011-1-AP)

    IHC staining of human placenta using 18011-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18011-1-AP (NUBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using NUBP1 Polyclonal antibody (18011-1-AP)

    IHC staining of human renal cell carcinoma using 18011-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 18011-1-AP (NUBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using NUBP1 Polyclonal antibody (18011-1-AP)

    IHC staining of mouse lung using 18011-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 18011-1-AP (NUBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using NUBP1 Polyclonal antibody (18011-1-AP)

    IHC staining of rat kidney using 18011-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 18011-1-AP (NUBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

NUBP1 Polyclonal antibody

NUBP1 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 18011-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Synonyms

Cytosolic Fe-S cluster assembly factor NUBP1, NBP, NBP 1, NBP1, Nbp35

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HeLa cells, LNCaP cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman stomach tissue, mouse kidney tissue, human kidney tissue, mouse lung tissue, rat kidney tissue, human renal cell carcinoma tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

18011-1-AP targets NUBP1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12510

Product name: Recombinant human NUBP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-309 aa of BC100290

Sequence: MEEVPHDCPGADSAQAGRGASCQGCPNQRLCASGAGATPDTAIEEIKEKMKTVKHKILVLSGKGGVGKSTFSAHLAHGLAEDENTQIALLDIDICGPSIPKIMGLEGEQYVEDNLGVMSVGFLLSSPDDAVIWRGPKKNGMIKQFLRDVDWGEVDYLIVDTPPGTSDEHLSVVRYLATAHIDGAVIITTPQEVSLQDVRKEINFCRKVKLPIIGVVENMSGFICPKCKKESQIFPPTTGGAELMCQDLEVPLLGRVPLDPLIGKNCDKGQSFFIDAPDSPATLAYRSIIQRIQEFCNLHQSKEENLISS

相同性解析による交差性が予測される生物種
Full Name nucleotide binding protein 1 (MinD homolog, E. coli)
Calculated molecular weight 309 aa, 33 kDa
Observed molecular weight36-40 kDa
GenBank accession numberBC100290
Gene Symbol NUBP1
Gene ID (NCBI) 4682
RRIDAB_2935446
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP53384
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for NUBP1 antibody 18011-1-APDownload protocol
IP protocol for NUBP1 antibody 18011-1-APDownload protocol
WB protocol for NUBP1 antibody 18011-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Adv

Autophagy supports mitochondrial metabolism through the regulation of iron homeostasis in pancreatic cancer

Authors - Subhadip Mukhopadhyay
View Article
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