Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells |
| Positive IHC detected in | rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
17566-1-AP targets CEP41 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11764 Product name: Recombinant human TSGA14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-301 aa of BC056162 Sequence: MSLRRHIGNPEYLMKRIPQNPRYQHIKSRLDTGNSMTKYTEKLEEIKKNYRYKKDELFKRLKVTTFAQLIIQVASLSDQTLEVTAEEIQRLEDNDSAASDPDAETTARTNGKGNPGEQSPSPEQFINNAGAGDSSRSTLQSVISGVGELDLDKGPVKKAEPHTKDKPYPDCPFLLLDVRDRDSYQQCHIVGAYSYPIATLSRTMNPYSNDILEYKNAHGKIIILYDDDERLASQAATTMCERGFENLFMLSGGRLNQANSSGRESKVPGARSAQNLPGGGPASHSNPRSLSSGHLQGKPWK 相同性解析による交差性が予測される生物種 |
| Full Name | testis specific, 14 |
| Calculated molecular weight | 301aa,33 kDa; 373aa,41 kDa |
| Observed molecular weight | 45 kDa |
| GenBank accession number | BC056162 |
| Gene Symbol | TSGA14 |
| Gene ID (NCBI) | 95681 |
| RRID | AB_2878417 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BYV8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TSGA14 (testis-specific Gene A14), also known as CEP41 (centrosomal protein of 41 kDa) , is a centrosomal protein identified in a mass-spectrometry-based proteomic analysis of human centrosomes. Mutations in the TSGA14 gene have been identified in families with autism spectrum disorders. (PMID:21438139)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CEP41 antibody 17566-1-AP | Download protocol |
| WB protocol for CEP41 antibody 17566-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Psychiatry ASD mutations in the ciliary gene CEP41 impact development of projection neurons and interneurons in a human cortical organoid model. | ||
J Cell Sci CEP41, a ciliopathy-linked centrosomal protein, regulates microtubule assembly and cell proliferation
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