SPAG11A Polyclonal antibody

SPAG11A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16770-1-AP

Synonyms

EP2, HE2, Protein EP2, SPAG11A, Sperm antigen HE2, sperm associated antigen 11A



Tested Applications

Positive WB detected inhuman testis tissue, human brain tissue
Positive IHC detected inhuman testis tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16770-1-AP targets SPAG11A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPAG11A fusion protein Ag9846
Full Name sperm associated antigen 11A
Calculated Molecular Weight 123 aa, 14 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC058833
Gene Symbol SPAG11A
Gene ID (NCBI) 653423
RRIDAB_2286582
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sperm binding proteins and their C-terminal peptides of the Sperm AssociatedAntigen 11 (SPAG11) family are found to play an important role in epididymal innate immunity in addition to their role in sperm maturation(PMID:16643671).SPAG11 peptides resulting due to cleavage at the furin-like proprotein convertase motif are reported in the epididymal fluid, ejaculate and on sperm and their antimicrobial activity demonstrated.SPAG11A belongs to this family and is also named as EP2,HE2. It has 4 isoforms produced by alternative splicing.This antibody is specific to SPAG11.

Protocols

Product Specific Protocols
WB protocol for SPAG11A antibody 16770-1-APDownload protocol
IHC protocol for SPAG11A antibody 16770-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

Reprod Biol Endocrinol

Sperm-associated antigen 11A is expressed exclusively in the principal cells of the mouse caput epididymis in an androgen-dependent manner.

Authors - Pujianto Dwi A DA