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  • Featured Product
  • KD/KO Validated

MMS19 Polyclonal antibody

MMS19 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16015-1-AP

Synonyms

hMMS19, MET18, MET18 homolog, MMS19, MMS19 like protein, MMS19L

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MMS19 Polyclonal antibody (16015-1-AP)

    WB analysis using 16015-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16015-1-AP (MMS19 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using MMS19 Polyclonal antibody (16015-1-AP)

    WB analysis of human brain using 16015-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16015-1-AP (MMS19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MMS19 Polyclonal antibody (16015-1-AP)

    IF Staining of HeLa using 16015-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16015-1-AP (MMS19 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, human brain tissue, PC-3 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16015-1-AP targets MMS19 in WB, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MMS19 fusion protein Ag8792
Full Name MMS19 nucleotide excision repair homolog (S. cerevisiae)
Calculated Molecular Weight 1030 aa, 113 kDa
Observed Molecular Weight 103-113 kDa
GenBank Accession NumberBC006575
Gene Symbol MMS19
Gene ID (NCBI) 64210
RRIDAB_2145043
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MMS19 plays a critical role in the biogenesis of multiple Fe-S proteins, which functions in genomic stability, RNA polymerase II function, and telomere length regulation. MMS19 has a alanine- and leucine-rich N terminus. It possible has a role in nucleotide excision repair(NER) and RNA polymerase II(POL II) transcription by interacting with ERCC2/XPB helicases, both subunits of NER-transcription factor TFIIH. Also MMS19 involves in the regulation of ER. It can function in chromosome segregation by acting as a part of the mitotic spindle-associated MMXD complex.

Protocols

Product Specific Protocols
WB protocol for MMS19 antibody 16015-1-APDownload protocol
IF protocol for MMS19 antibody 16015-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

Hypoxia Rescues Frataxin Loss by Restoring Iron Sulfur Cluster Biogenesis.

Authors - Tslil Ast
View Article
humanWB

Mol Cell

Cytosolic Iron-Sulfur Assembly Is Evolutionarily Tuned by a Cancer-Amplified Ubiquitin Ligase.

Authors - Jenny L Weon
  • KD Validated
View Article
humanWB

Cell Metab

Human CIA2A-FAM96A and CIA2B-FAM96B Integrate Iron Homeostasis and Maturation of Different Subsets of Cytosolic-Nuclear Iron-Sulfur Proteins.

Authors - Stehling Oliver O
  • KD Validated
View Article
mouseWB

Nat Commun

Structural basis for substrate recognition and chemical inhibition of oncogenic MAGE ubiquitin ligases.

Authors - Seung Wook Yang
View Article
humanWB

Cell Rep

The CIA Targeting Complex Is Highly Regulated and Provides Two Distinct Binding Sites for Client Iron-Sulfur Proteins.

Authors - Diana C Odermatt
  • KD Validated
View Article
mouseWB

Mol Metab

Cdkal1, a type 2 diabetes susceptibility gene, regulates mitochondrial function in adipose tissue.

Authors - Colin J Palmer
View Article