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  • Featured Product
  • KD/KO Validated

SAR1A Polyclonal antibody

SAR1A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15350-1-AP

Synonyms

COPII associated small GTPase, GTP binding protein SAR1a, masra2, SAR1, SAR1 homolog A (S. cerevisiae), SAR1A, Sara, SARA1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using SAR1A Polyclonal antibody (15350-1-AP)

    WB analysis of mouse liver using 15350-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15350-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using SAR1A Polyclonal antibody (15350-1-AP)

    WB analysis of HT-1080 using 15350-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 15350-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using SAR1A Polyclonal antibody (15350-1-AP)

    WB analysis of HT-1080 using 15350-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 15350-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SAR1A Polyclonal antibody (15350-1-AP)

    WB analysis of HeLa using 15350-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15350-1-AP (SAR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using SAR1A Polyclonal antibody (15350-1-AP)

    WB analysis of mouse liver using 15350-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15350-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using SAR1A Polyclonal antibody (15350-1-AP)

    IHC staining of human hepatocirrhosis using 15350-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15350-1-AP (SAR1A antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using SAR1A Polyclonal antibody (15350-1-AP)

    IHC staining of human hepatocirrhosis using 15350-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15350-1-AP (SAR1A antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using SAR1A Polyclonal antibody (15350-1-AP)

    IHC staining of human liver using 15350-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15350-1-AP (SAR1A Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using SAR1A Polyclonal antibody (15350-1-AP)

    IHC staining of human liver using 15350-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15350-1-AP (SAR1A Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using SAR1A Polyclonal antibody (15350-1-AP)

    IHC staining of human skeletal muscle using 15350-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15350-1-AP (SAR1A Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using SAR1A Polyclonal antibody (15350-1-AP)

    IHC staining of human skeletal muscle using 15350-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15350-1-AP (SAR1A Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse liver tissue, HeLa cells, HT-1080 cells
Positive IHC detected inhuman hepatocirrhosis tissue, human liver tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15350-1-AP targets SAR1A in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SAR1A fusion protein Ag7596
Full Name SAR1 homolog A (S. cerevisiae)
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 23-25 kDa
GenBank Accession NumberBC003658
Gene Symbol SAR1A
Gene ID (NCBI) 56681
RRIDAB_10667406
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SAR1A antibody 15350-1-APDownload protocol
IHC protocol for SAR1A antibody 15350-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Clin Invest

Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.

Authors - Zhixiong Liu
View Article
human,mouseWB

Redox Biol

Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis

Authors - Mengyue You
View Article
humanWB

Proc Natl Acad Sci U S A

Atlastin-mediated membrane tethering is critical for cargo mobility and exit from the endoplasmic reticulum.

Authors - Liling Niu
View Article
ratWB

J Biol Chem

The Endoplasmic Reticulum COPII-Vesicle Transport Machinery Coordinates Cellular Lipid Secretion and Cholesterol Biosynthesis.

Authors - Lee G D Fryer
View Article
human,mouseIHC,WB

Clin Epigenetics

Epigenetic silencing of TMEM176A activates ERK signaling in human hepatocellular carcinoma.

Authors - Hongxia Li
  • KD Validated
View Article
humanWB,IP

Biochem Biophys Res Commun

Pull down assay for GTP-bound form of Sar1a reveals its activation during morphological differentiation.

Authors - Yuri Urai
View Article