Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of HeLa using 15204-1-AP
WB result of CHOP antibody (15204-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHOP transfected HeLa cells.
WB analysis using 15204-1-AP
Tunicamycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 15204-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 15204-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 15204-1-AP (CHOP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of C6 using 15204-1-AP
IP result of anti-CHOP; GADD153 (IP:15204-1-AP, 4ug; Detection:15204-1-AP 1:4000) with C6 cells lysate 1600 ug.
IHC staining of human breast cancer using 15204-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 15204-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 15204-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 15204-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 15204-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 15204-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human thyroid cancer using 15204-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human thyroid cancer using 15204-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 15204-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 15204-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 15204-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 15204-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 15204-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of U2OS using 15204-1-AP
Immunofluorescent analysis of (4% PFA) fixed thapsigargin treated U2OS cells using CHOP/GADD153 antibody (15204-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), F-actin (orange), GRP78/BIP antibody (66574-1-Ig, Clone: 1D6F7, Magenta).
IF Staining of U2OS using 15204-1-AP
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CHOP/GADD153 antibody (15204-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), GRP78/BIP antibody (66574-1-Ig, Clone: 1D6F7, Magenta), F-actin (orange).
IF Staining of HeLa using 15204-1-AP
Immunofluorescent analysis of (4% PFA) fixed Tunicamycin treated HeLa cells using CHOP; GADD153 antibody (15204-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
FC experiment of HeLa using 15204-1-AP
1X10^6 HeLa cells untreated (dashed lines) or treated with Tunicamycin (20 μg/mL, 4 h; red) were intracellularly stained with 0.4 ug Anti-Human CHOP; GADD153 (15204-1-AP) and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.4 ug Control Antibody (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Featured Product
KD/KO Validated

CHOP/GADD153 Polyclonal antibody

CHOP/GADD153 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 15204-1-AP

Publications(888)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA

Synonyms

CHOP; GADD153, CHOP, DDIT3, GADD153, C/EBP zeta

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (888)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Tunicamycin treated HeLa cells, MCF-7 cells, HeLa cells, K-562 cells, RAW 264.7 cells
Positive IP detected in	C6 cells
Positive IHC detected in	human colon cancer tissue, human breast cancer tissue, human thyroid cancer tissue, human cervical cancer tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Tunicamycin treated HeLa cells, thapsigargin treated U2OS cells
Positive FC (Intra) detected in	Tunicamycin treated HeLa cells

Samples need to be treated with ER stress.

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 37 publications below
WB	See 763 publications below
IHC	See 99 publications below
IF	See 111 publications below
IP	See 6 publications below
CoIP	See 2 publications below
ChIP	See 2 publications below

Product Information

15204-1-AP targets CHOP/GADD153 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, rabbit, canine, chicken, bovine, hamster
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7354 Product name: Recombinant human CHOP; GADD153 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-169 aa of BC003637 Sequence: MAAESLPFSFGTLSSWELEAWYEDLQEVLSSDENGGTYVSPPGNEEEESKIFTTLDPASLAWLTEEEPEPAEVTSTSQSPHSPDSSQSSLAQEEEEEDQGRTRKRKQSGHSPARAGKQRMKEKEQENERKVAQLAEENERLKQEIERLTREVEATRRALIDRMVNLHQA 相同性解析による交差性が予測される生物種
Full Name	DNA-damage-inducible transcript 3
Calculated molecular weight	19 kDa
Observed molecular weight	30 kDa
GenBank accession number	BC003637
Gene Symbol	CHOP
Gene ID (NCBI)	1649
RRID	AB_2292610
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P35638
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).

Protocols

Product Specific Protocols
IF protocol for CHOP/GADD153 antibody 15204-1-AP	Download protocol
IHC protocol for CHOP/GADD153 antibody 15204-1-AP	Download protocol
IP protocol for CHOP/GADD153 antibody 15204-1-AP	Download protocol
WB protocol for CHOP/GADD153 antibody 15204-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Nature Ageing promotes metastasis via activation of the integrated stress response Authors - Angana A H Patel View Article
human	WB	Science Endosomal lipid signaling reshapes the endoplasmic reticulum to control mitochondrial function Authors - Wonyul Jang View Article
mouse	IF	Cell ER stress transforms random olfactory receptor choice into axon targeting precision Authors - Hani J Shayya View Article
mouse	WB	Nat Biotechnol Programmable initiation of mRNA translation by trans-RNA Authors - Longfei Jia View Article
rat	IHC	Adv Mater Poly(Citric Acid) Bidirectional Regulator: Coordinating Iron Homeostasis to Suppress Stress Responses and Boosting Mitochondrial Bioenergetics for Enhanced Nerve Repair. Authors - Xinyue Liang View Article
human	WB	Cancer Commun (Lond) HMMR alleviates endoplasmic reticulum stress by promoting autophagolysosomal activity during endoplasmic reticulum stress-driven hepatocellular carcinoma progression Authors - Lin He View Article

Validation Data Gallery

WB analysis of HeLa using 15204-1-AP

WB analysis using 15204-1-AP

WB analysis using 15204-1-AP

WB analysis of MCF-7 using 15204-1-AP

IP experiment of C6 using 15204-1-AP

IHC staining of human breast cancer using 15204-1-AP

IHC staining of human colon cancer using 15204-1-AP

IHC staining of human colon cancer using 15204-1-AP

IHC staining of human cervical cancer using 15204-1-AP

IHC staining of human breast cancer using 15204-1-AP

IHC staining of human colon cancer using 15204-1-AP

IHC staining of human thyroid cancer using 15204-1-AP

IHC staining of human thyroid cancer using 15204-1-AP

IHC staining of human cervical cancer using 15204-1-AP

IHC staining of human cervical cancer using 15204-1-AP

IHC staining of mouse brain using 15204-1-AP

IHC staining of mouse brain using 15204-1-AP

IHC staining of human breast cancer using 15204-1-AP

IF Staining of U2OS using 15204-1-AP

IF Staining of U2OS using 15204-1-AP

IF Staining of HeLa using 15204-1-AP

FC experiment of HeLa using 15204-1-AP

CHOP/GADD153 Polyclonal antibody

CHOP/GADD153 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Samples need to be treated with ER stress.

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Ageing promotes metastasis via activation of the integrated stress response

Endosomal lipid signaling reshapes the endoplasmic reticulum to control mitochondrial function

ER stress transforms random olfactory receptor choice into axon targeting precision

Programmable initiation of mRNA translation by trans-RNA

Poly(Citric Acid) Bidirectional Regulator: Coordinating Iron Homeostasis to Suppress Stress Responses and Boosting Mitochondrial Bioenergetics for Enhanced Nerve Repair.

HMMR alleviates endoplasmic reticulum stress by promoting autophagolysosomal activity during endoplasmic reticulum stress-driven hepatocellular carcinoma progression

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