Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, HuH-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14158-1-AP targets MIPOL1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5340 Product name: Recombinant human MIPOL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-261 aa of BC035870 Sequence: MSRLQLAIEERDEAIARAKHMEMSLKVLENINPEENDMTLQELLNRINNADTGIAIQKNGAIIVDRIYKTKECKMRITAEEMSALIEERDAALSKCKRLEQELHHVKEQNQTSANNMRHLTAENNQERALKAKLLSMQQARETAVQQYKKLEEEIQTLRVYYSLHKSLSQEENLKDQFNYTLSTYEEALKNRENIVSITQQQNEELATQLQQALTERANMELQLQHAREASQVANEKVQKLERLVDVLRKKVGTGTMRTVI 相同性解析による交差性が予測される生物種 |
| Full Name | mirror-image polydactyly 1 |
| Calculated molecular weight | 442 aa, 52 kDa |
| Observed molecular weight | 52 kDa |
| GenBank accession number | BC035870 |
| Gene Symbol | MIPOL1 |
| Gene ID (NCBI) | 145282 |
| RRID | AB_2141930 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TD10 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MIPOL1 (also named CCDC193) encodes a nuclear coiled-coil protein. Germline MIPOL1 variants cause Laurin‑Sandrow syndrome characterized by mirror-image polydactyly. It acts as a candidate tumor suppressor in nasopharyngeal carcinoma. Downregulation of MIPOL1 activates AKT/FAK signaling, elevates angiogenic cytokines, and promotes cancer invasion and metastasis. Restoration of wild-type MIPOL1 enhances RhoB activity and suppresses tumor progression both in vitro and in xenograft mouse models. (PMID: 31609475)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MIPOL1 antibody 14158-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |