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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-18BP Polyclonal antibody (14153-1-AP)

    WB analysis using 14153-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14153-1-AP (IL-18BP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using IL-18BP Polyclonal antibody (14153-1-AP)

    WB analysis of A549 using 14153-1-AP

    WB result of IL-18BP antibody (14153-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IL-18BP transfected A549 cells.

  • Featured Product
  • KD/KO Validated

IL-18BP Polyclonal antibody

IL-18BP Polyclonal Antibody for WB, ELISA
Cat No. 14153-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Synonyms

IL18BP, IL 18BP, IL18BPa, interleukin 18 binding protein, Interleukin-18-binding protein

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

14153-1-AP targets IL-18BP in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5335

Product name: Recombinant human IL-18BP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 30-194 aa of BC044215

Sequence: ATPVSQTTTAATASVRSTKDPCPSQPPVFPAAKQCPALEVTWPEVEVPLNGTLSLSCVACSRFPNFSILYWLGNGSFIEHLPGRLWEGSTSRERGSTGTQLCKALVLEQLTPALHSTNFSCVLVDPEQVVQRHVVLAQLWAGLRATLPPTQEALPSSHSSPQQQG

相同性解析による交差性が予測される生物種
Full Name interleukin 18 binding protein
Calculated molecular weight 194 aa, 21 kDa
Observed molecular weight21 kDa
GenBank accession numberBC044215
Gene Symbol IL-18BP
Gene ID (NCBI) 10068
RRIDAB_2918034
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO95998
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL18BP, also named as adekinig-alfa, has 3 isoforms (Isoform A 21kd; B 12kd and D 17kd). Isoform A binds to IL-18 and inhibits its activity. IL18BP functions as an inhibitor of the early TH1 cytokine response. Highly glycosylated of IL18BP will migrate to be 40kd. A 58kd protein is IL18BP crosslinked IL18.

Protocols

Product Specific Protocols
WB protocol for IL-18BP antibody 14153-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Bioact Mater

Bone targeted microenvironment actuated engineered exosomes for precision therapy of IBD associated bone loss.

Authors - Jiang Liu
View Article
mouseWB

Front Microbiol

Proteus mirabilis exacerbates ulcerative colitis by inhibiting mucin production

Authors - Zhihui Jiang
View Article
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