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SLC7A5 Polyclonal antibody
SLC7A5 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 13752-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse lung tissue, human placenta tissue, MCF-7 cells |
Positive IHC detected in | human lung tissue, mouse lung tissue, mouse liver tissue, human testis tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 11 publications below |
IHC | See 6 publications below |
IF | See 4 publications below |
Product Information
13752-1-AP targets SLC7A5 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, cow |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SLC7A5 fusion protein Ag4694 |
Full Name | solute carrier family 7 (cationic amino acid transporter, y+ system), member 5 |
Calculated Molecular Weight | 507 aa, 55 kDa |
Observed Molecular Weight | 45-50 kDa |
GenBank Accession Number | BC039692 |
Gene Symbol | SLC7A5 |
Gene ID (NCBI) | 8140 |
RRID | AB_2190973 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for SLC7A5 antibody 13752-1-AP | Download protocol |
IHC protocol for SLC7A5 antibody 13752-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Mol Life Sci Regulation of branched-chain amino acid metabolism by hypoxia-inducible factor in glioblastoma. | ||
ACS Chem Neurosci Baicalein Delays H2O2-Induced Astrocytic Senescence through Inhibition of Senescence-Associated Secretory Phenotype (SASP), Suppression of JAK2/STAT1/NF-κB Pathway, and Regulation of Leucine Metabolism. | ||
J Biol Chem GSE1 predicts poor survival outcome in gastric cancer patients by SLC7A5 enhancement of tumor growth and metastasis. | ||
Br J Cancer ASCT2 (SLC1A5)-dependent glutamine uptake is involved in the progression of head and neck squamous cell carcinoma. |