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  • KD/KO Validated

IFIT5 Polyclonal antibody

IFIT5 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13378-1-AP

Synonyms

IFIT 5, IFIT5, RI58



Tested Applications

Positive WB detected inA375 cells, PC-3 cells
Positive IP detected inA375 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13378-1-AP targets IFIT5 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, pig, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFIT5 fusion protein Ag4202
Full Name interferon-induced protein with tetratricopeptide repeats 5
Calculated Molecular Weight 482 aa, 58 kDa
Observed Molecular Weight 56-58 kDa, 48 kDa
GenBank Accession NumberBC025786
Gene Symbol IFIT5
Gene ID (NCBI) 24138
RRIDAB_10640300
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFIT5, also named as Interferon-induced 58 kDa protein or RI58, is a 482 amino acid protein, which contains eight TPR repeats and belongs to the IFIT family. IFIT5 localizes in the Cell projection. Interferon-induced RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs.

Protocols

Product Specific Protocols
WB protocol for IFIT5 antibody 13378-1-APDownload protocol
IHC protocol for IFIT5 antibody 13378-1-APDownload protocol
IP protocol for IFIT5 antibody 13378-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Immunol

IFIT5 Negatively Regulates the Type I IFN Pathway by Disrupting TBK1-IKKε-IRF3 Signalosome and Degrading IRF3 and IKKε.

Authors - Na Zhang
humanWB

Cell Signal

IFIT5 positively regulates NF-κB signaling through synergizing the recruitment of IκB kinase (IKK) to TGF-β-activated kinase 1 (TAK1).

Authors - Caishang Zheng
  • KD Validated
HumanWB

Acta Biochim Biophys Sin (Shanghai)

IFIT5 potentiates anti-viral response through enhancing innate immune signaling pathways.

Authors - Zhang Bianhong B
humanWB,IHC

Cell Death Dis

The roles and mechanism of IFIT5 in bladder cancer epithelial-mesenchymal transition and progression.

Authors - Jun Huang
humanIHC

Oncoimmunology

Development and validation of an interferon signature predicting prognosis and treatment response for glioblastoma.

Authors - Chen Zhu
humanWB

mBio

PKR Binds Enterovirus IRESs, Displaces Host Translation Factors, and Impairs Viral Translation to Enable Innate Antiviral Signaling.

Authors - Mikhail I Dobrikov