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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using KLK 11 Polyclonal antibody (12698-1-AP)

    WB analysis of MCF-7 using 12698-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12698-1-AP (KLK 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using KLK 11 Polyclonal antibody (12698-1-AP)

    WB analysis of HeLa using 12698-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12698-1-AP (KLK 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using KLK 11 Polyclonal antibody (12698-1-AP)

    WB analysis of PC-3 using 12698-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12698-1-AP (KLK 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using KLK 11 Polyclonal antibody (12698-1-AP)

    WB analysis of Y79 using 12698-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 12698-1-AP (KLK 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using KLK 11 Polyclonal antibody (12698-1-AP)

    IHC staining of human prostate cancer using 12698-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12698-1-AP (KLK 11 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using KLK 11 Polyclonal antibody (12698-1-AP)

    IHC staining of human prostate cancer using 12698-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12698-1-AP (KLK 11 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using KLK 11 Polyclonal antibody (12698-1-AP)

    IHC staining of human brain using 12698-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 12698-1-AP (KLK 11 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using KLK 11 Polyclonal antibody (12698-1-AP)

    IHC staining of human prostate cancer using 12698-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12698-1-AP (KLK 11 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using KLK 11 Polyclonal antibody (12698-1-AP)

    IHC staining of human brain using 12698-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 12698-1-AP (KLK 11 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using KLK 11 Polyclonal antibody (12698-1-AP)

    IF Staining of PC-3 using 12698-1-AP

    Immunofluorescent analysis of PC-3 cells using 12698-1-AP (KLK 11 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

KLK 11 Polyclonal antibody

KLK 11 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12698-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Kallikrein 11, KLK11, EC:3.4.21.-, Kallikrein11, Kallikrein-11

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF7 cells, HeLa cells, PC-3 cells, Y79 cells
Positive IHC detected inhuman prostate cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12698-1-AP targets KLK 11 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3427

Product name: Recombinant human KLK11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 58-250 aa of BC022068

Sequence: LTAAHCLKPRYIVHLGQHNLQKEEGCEQTRTATESFPHPGFNNSLPNKDHRNDIMLVKMASPVSITWAVRPLTLSSRCVTAGTSCLISGWGSTSSPQLRLPHTLRCANITIIEHQKCENAYPGNITDTMVCASVQEGGKDSCQGDSGGPLVCNQSLQGIISWGQDPCAITRKPGVYTKVCKYVDWIQETMKNN

相同性解析による交差性が予測される生物種
Full Name kallikrein-related peptidase 11
Calculated molecular weight 250 aa, 27 kDa
Observed molecular weight 30-40 kDa
GenBank accession numberBC022068
Gene Symbol KLK11
Gene ID (NCBI) 11012
RRIDAB_2877876
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBX7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KLK11(Kallikrein-11) is also named as PRSS20, TLSP and belongs to the peptidase S1 family and Kallikrein subfamily. It is a multifunctinal protease cleaving several substrates for kallikrein and trypsin and involved in normal physiology processes in bronchus. This full length protein has a signal peptide, a propeptide and four glycosylation sites. It has 4 isoforms produced by alternative splicing with the molecular weight of 31 kDa, 27 kDa,34 kDa and 30 kDa.

Protocols

Product Specific Protocols
IF protocol for KLK 11 antibody 12698-1-APDownload protocol
IHC protocol for KLK 11 antibody 12698-1-APDownload protocol
WB protocol for KLK 11 antibody 12698-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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