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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPP2R1B Polyclonal antibody (12621-1-AP)

    WB analysis using 12621-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12621-1-AP (PPP2R1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PPP2R1B Polyclonal antibody (12621-1-AP)

    WB analysis using 12621-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12621-1-AP (PPP2R1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using PPP2R1B Polyclonal antibody (12621-1-AP)

    WB analysis of human kidney using 12621-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12621-1-AP (PPP2R1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using PPP2R1B Polyclonal antibody (12621-1-AP)

    IHC staining of human liver using 12621-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12621-1-AP (PPP2R1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using PPP2R1B Polyclonal antibody (12621-1-AP)

    IHC staining of human liver using 12621-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12621-1-AP (PPP2R1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using PPP2R1B Polyclonal antibody (12621-1-AP)

    IF Staining of Caco-2 using 12621-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using PPP2R1B antibody (12621-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

PPP2R1B Polyclonal antibody

PPP2R1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12621-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Synonyms

Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform, PR65B, PP2A subunit A isoform R1-beta, PP2A subunit A isoform R1 beta, PP2A subunit A isoform PR65-beta

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA2780 cells, human kidney tissue, mouse testis tissue, rat testis tissue, HeLa cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCaco-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12621-1-AP targets PPP2R1B in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3307

Product name: Recombinant human PPP2R1B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 320-667 aa of BC027596

Sequence: ELGENLPIEDRETIIMNQILPYIKELVSDTNQHVKSALASVIMGLSTILGKENTIEHLLPLFLAQLKDECPDVRLNIISNLDCVNEVIGIRQLSQSLLPAIVELAEDAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATNNLMKLVQKFGTEWAQNTIVPKVLVMANDPNYLHRMTTLFCINALSEACGQEITTKQMLPIVLKMAGDQVANVRFNVAKSLQKIGPILDTNALQGEVKPVLQKLGQDEDMDVKYFAQEAISVVAQRLRKLEFPVKDSGEPSVPRADKNHFPRPTVPGEDMGKGPVYQLRGDTRDTLAQLGIAELVHFSQSTD

相同性解析による交差性が予測される生物種
Full Name protein phosphatase 2 (formerly 2A), regulatory subunit A, beta isoform
Calculated molecular weight 667 aa, 74 kDa
Observed molecular weight 60-74 kDa
GenBank accession numberBC027596
Gene Symbol PPP2R1B
Gene ID (NCBI) 5519
RRIDAB_2169941
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP30154
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for PPP2R1B antibody 12621-1-APDownload protocol
IHC protocol for PPP2R1B antibody 12621-1-APDownload protocol
WB protocol for PPP2R1B antibody 12621-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC,CoIP

Cancer Cell Int

PPP2R1B abolishes colorectal cancer liver metastasis and sensitizes Oxaliplatin by inhibiting MAPK/ERK signaling pathway

Authors - Wei Liu
  • KD Validated
View Article
mouseWB

EMBO Rep

CIP2A deficiency promotes depression-like behaviors in mice through inhibition of dendritic arborization

Authors - Wen-Ting Hu
View Article
humanWB

Front Cell Dev Biol

BRG1 Activates PR65A Transcription to Regulate NO Bioavailability in Vascular Endothelial Cells.

Authors - Baoyu Chen
View Article
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