Validation Data Gallery
Tested Applications
| Positive WB detected in | SH-SY5Y cells, HeLa cells, human brain tissue, PC-3 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
Product Information
11928-1-AP targets TMEFF2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2609 Product name: Recombinant human TMEFF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-374 aa of BC008973 Sequence: MVLWESPRQCSSWTLCEGFCWLLLLPVMLLIVARPVKLAAFPTSLSDCQTPTGWNCSGYDDRENDLFLCDTNTCKFDGECLRIGDTVTCVCQFKCNNDYVPVCGSNGESYQNECYLRQAACKQQSEILVVSEGSCATDAGSGSGDGVHEGSGETSQKETSTCDICQFGAECDEDAEDVWCVCNIDCSQTNFNPLCASDGKSYDNACQIKEASCQKQEKIEVMSLGRCQDNTTTTTKSEDGHYARTDYAENANKLEESAREHHIPCPEHYNGFCMHGKCEHSINMQEPSCRCDAGYTGQHCEKKDYSVLYVVPGPVRFQYVLIAAVIGTIQIAVICVVVLCITRKCPRSNRIHRQKQNTGHYSSDNTTRASTRLI 相同性解析による交差性が予測される生物種 |
| Full Name | transmembrane protein with EGF-like and two follistatin-like domains 2 |
| Calculated molecular weight | 374 aa, 41 kDa |
| Observed molecular weight | 41 kDa |
| GenBank accession number | BC008973 |
| Gene Symbol | TMEFF2 |
| Gene ID (NCBI) | 23671 |
| RRID | AB_2204477 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UIK5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TMEFF2 antibody 11928-1-AP | Download protocol |
| WB protocol for TMEFF2 antibody 11928-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Immunol Gene expression profiling and real-time PCR analyses identify novel potential cancer-testis antigens in multiple myeloma. | ||
Cancer Cell Int TMEFF2 promoter hypermethylation is an unfavorable prognostic marker in gliomas. |





