Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 11685-1-AP
U2OS (positive), Jurkat, Ramos, and U-937 (negative) cells were subjected to SDS PAGE followed by western blot with 11685-1-AP (PLEK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 11685-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11685-1-AP (PLEK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Caco-2 using 11685-1-AP
Caco-2 cells were subjected to SDS PAGE followed by western blot with 11685-1-AP (PLEK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HT-29 using 11685-1-AP
IP result of anti-PLEK2 (IP:11685-1-AP, 4ug; Detection:11685-1-AP 1:1000) with HT-29 cells lysate 1760 ug.
IHC staining of human pancreas cancer using 11685-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11685-1-AP (PLEK2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of mouse spleen using 11685-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11685-1-AP (PLEK2 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of mouse spleen using 11685-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11685-1-AP (PLEK2 Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of HT-29 using 11685-1-AP
Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using PLEK2 antibody (11685-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HT-29 using 11685-1-AP
1X10^6 HT-29 cells were intracellularly stained with 0.4 ug Anti-Human PLEK2 (11685-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

PLEK2 Polyclonal antibody

PLEK2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 11685-1-AP

Publications(22)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

Pleckstrin 2, Pleckstrin-2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (22)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HT-29 cells, U2OS cells, Caco-2 cells, PC-3 cells
Positive IP detected in	HT-29 cells
Positive IHC detected in	human pancreas cancer tissue, mouse spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HT-29 cells
Positive FC (Intra) detected in	HT-29 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 12 publications below
WB	See 19 publications below
IHC	See 9 publications below
IF	See 3 publications below
IP	See 1 publications below
CoIP	See 2 publications below

Product Information

11685-1-AP targets PLEK2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2297 Product name: Recombinant human PLEK2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 90-353 aa of BC001226 Sequence: EERDAWAFEITGAIHAGQPGKVQQLHSLRNSFKLPPHISLHRIVDKMHDSNTGIRSSPNMEQGSTYKKTFLGSSLVDWLISNSFTASRLEAVTLASMLMEENFLRPVGVRSMGAIRSGDLAEQFLDDSTALYTFAESYKKKISPKEEISLSTVELSGTVVKQGYLAKQGHKRKNWKVRRFVLRKDPAFLHYYDPSKEENRPVGGFSLRGSLVSALEDNGVPTGVKGNVQGNLFKVITKDDTHYYIQASSKAERAEWIEAIKKLT 相同性解析による交差性が予測される生物種
Full Name	pleckstrin 2
Calculated molecular weight	353 aa, 40 kDa
Observed molecular weight	38-40 kDa
GenBank accession number	BC001226
Gene Symbol	PLEK2
Gene ID (NCBI)	26499
RRID	AB_2252364
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NYT0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PLEK2 belongs to the Pleckstrin homology domain family and is a 40-kDa protein containing the prototypic PH domains at its amino and carboxyl termini(PMID: 10419454). PLEK2 associates with membrane-bound phosphatidylinositols generated by phosphatidylinositol 3-kinase. PLEK2 interacts with the actin cytoskeleton to induce cell spreading.

Protocols

Product Specific Protocols
FC protocol for PLEK2 antibody 11685-1-AP	Download protocol
IF protocol for PLEK2 antibody 11685-1-AP	Download protocol
IHC protocol for PLEK2 antibody 11685-1-AP	Download protocol
IP protocol for PLEK2 antibody 11685-1-AP	Download protocol
WB protocol for PLEK2 antibody 11685-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse,human	WB	Cancer Commun (Lond) PLEK2 promotes cancer stemness and tumorigenesis of head and neck squamous cell carcinoma via the c-Myc-mediated positive feedback loop Authors - Xinyuan Zhao KD Validated View Article
human	IHC,CoIP	J Exp Clin Cancer Res PLEK2 promotes gallbladder cancer invasion and metastasis through EGFR/CCL2 pathway. Authors - Hui Shen View Article
mouse	WB,IP	J Clin Invest Targeting pleckstrin-2-Akt signaling reduces proliferation in myeloproliferative neoplasm models Authors - Xu Han View Article
mouse	WB,IHC	J Clin Invest Loss of pleckstrin-2 reverts lethality and vascular occlusions in JAK2V617F-positive myeloproliferative neoplasms. Authors - Baobing Zhao KO Validated View Article
human	WB	Mol Biomed Unveiling the role of Pleckstrin-2 in tumor progression and immune modulation: insights from a comprehensive pan-cancer analysis with focus on lung cancer Authors - Enzhi Yin KD Validated View Article
human	WB,IHC	Cell Death Dis TGF-β-induced PLEK2 promotes metastasis and chemoresistance in oesophageal squamous cell carcinoma by regulating LCN2. Authors - Feng Wang KD Validated View Article

Validation Data Gallery

WB analysis using 11685-1-AP

WB analysis using 11685-1-AP

WB analysis of Caco-2 using 11685-1-AP

IP experiment of HT-29 using 11685-1-AP

IHC staining of human pancreas cancer using 11685-1-AP

IHC staining of mouse spleen using 11685-1-AP

IHC staining of mouse spleen using 11685-1-AP

IF Staining of HT-29 using 11685-1-AP

FC experiment of HT-29 using 11685-1-AP

PLEK2 Polyclonal antibody

PLEK2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

PLEK2 promotes cancer stemness and tumorigenesis of head and neck squamous cell carcinoma via the c-Myc-mediated positive feedback loop

PLEK2 promotes gallbladder cancer invasion and metastasis through EGFR/CCL2 pathway.

Targeting pleckstrin-2-Akt signaling reduces proliferation in myeloproliferative neoplasm models

Loss of pleckstrin-2 reverts lethality and vascular occlusions in JAK2V617F-positive myeloproliferative neoplasms.

Unveiling the role of Pleckstrin-2 in tumor progression and immune modulation: insights from a comprehensive pan-cancer analysis with focus on lung cancer

TGF-β-induced PLEK2 promotes metastasis and chemoresistance in oesophageal squamous cell carcinoma by regulating LCN2.

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