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UCHL5 Polyclonal antibody

UCHL5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11527-1-AP

Synonyms

CGI 70, INO80R, Ubiquitin thioesterase L5, UCH L5, UCH37, UCHL5



Tested Applications

Positive WB detected inmouse colon tissue, mouse brain tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11527-1-AP targets UCHL5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UCHL5 fusion protein Ag2095
Full Name ubiquitin carboxyl-terminal hydrolase L5
Calculated Molecular Weight 326 aa, 37 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC025369
Gene Symbol UCHL5
Gene ID (NCBI) 51377
RRIDAB_2877774
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UCHL5 (Ubiquitin carboxyl-terminal hydrolase isozyme L5) is also named as UCH37 and belongs to the peptidase C12 family. It is functionally linked with multiple protein complexes and signal transduction pathways. UCH37's weight average molecular weight increases as a function of its concentration. By size-exclusion chromatography, the apparent molecular weight of UCH37 was 51, 56, 70, and 109 kDa at 0.15, 0.69, 2.4, and 9.5 mg/mL, respectively. Given UCH37's monomeric molecular weight of 37 kDa and its apparent elongated shape, its tendency toward an apparent molecular weight between 40 and 50 kDa at decreasing concentrations is appropriate for UCH37 monomers (PMID:21953935).

Protocols

Product Specific Protocols
WB protocol for UCHL5 antibody 11527-1-APDownload protocol
IHC protocol for UCHL5 antibody 11527-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Oncol

Ubiquitin C-Terminal Hydrolase L5 (UCHL5) Accelerates the Growth of Endometrial Cancer via Activating the Wnt/β-Catenin Signaling Pathway.

Authors - Da Liu
  • KD Validated
mouseWB

FASEB J

Caveolin-2 in association with nuclear lamina controls adipocyte hypertrophy

Authors - Moonjeong Choi
humanWB

J Cell Biochem

Computational and biochemical studies of isothiocyanates as inhibitors of proteasomal cysteine deubiquitinases in human cancer cells.

Authors - Zainab Sabry Othman Ahmed
humanWB

J Cell Biochem

Proteasome-associated cysteine deubiquitinases are molecular targets of environmental optical brightener compounds.

Authors - Isel Castro
humanIHC

Mol Ther Oncolytics

The UCHL5 inhibitor b-AP15 overcomes cisplatin resistance via suppression of cancer stemness in urothelial carcinoma

Authors - Po-Ming Chow
humanIHC

Am J Cancer Res

Anti-tumor effects of deubiquitinating enzyme inhibitor PR-619 in human chondrosarcoma through reduced cell proliferation and endoplasmic reticulum stress-related apoptosis

Authors - Wei-Chou Lin