Validation Data Gallery
Tested Applications
| Positive WB detected in | Raji cells |
| Positive IHC detected in | human gliomas tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 6 publications below |
| IF | See 2 publications below |
Product Information
11454-1-AP targets MZB1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2015 Product name: Recombinant human MZB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-189 aa of BC021275 Sequence: MRLSLPLLLLLLGAWAIPGGLGDRAPLTATAPQLDDEEMYSAHMPAHLRCDACRAVAYQMWQNLAKAETKLHTSNSGGRRELSELVYTDVLDRSCSRNWQDYGVREVDQVKRLTGPGLSEGPEPSISVMVTGGPWPTRLSRTCLHYLGEFGEDQIYEAHQQGRGALEALLCGGPQGACSEKVSATREEL 相同性解析による交差性が予測される生物種 |
| Full Name | hypothetical protein MGC29506 |
| Calculated molecular weight | 189 aa, 21 kDa |
| Observed molecular weight | 19-21 kDa |
| GenBank accession number | BC021275 |
| Gene Symbol | MZB1 |
| Gene ID (NCBI) | 51237 |
| RRID | AB_832820 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WU39 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MZB1, also named as ,PACAP, MGC29506 and pERp1, belongs to the PERP1 family. It is associates with immunoglobulin M (IgM) heavy and light chains and promotes IgM assembly and secretion. MGC29506(PACAP) may exert its effect by acting as a molecular chaperone or as an oxidoreductase as it displays a low level of oxidoreductase activity. Isoform 2 may be involved in regulation of apoptosis. (PubMed: 11350957) MZB1 has 5 isoforms with MW 21kd and 7-13kd.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MZB1 antibody 11454-1-AP | Download protocol |
| WB protocol for MZB1 antibody 11454-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Clin Cancer Res Integrative array-based approach identifies MZB1 as a frequently methylated putative tumor suppressor in hepatocellular carcinoma. | ||
J Pathol Proteomic profiling identifies novel diagnostic biomarkers and molecular subtypes for mucinous tubular and spindle cell carcinoma of the kidney. | ||
Int J Cancer Epigenetic suppression of the immunoregulator MZB1 is associated with the malignant phenotype of gastric cancer. | ||
J Biol Chem Transgenic expression of microRNA-185 causes a developmental arrest of T cells by targeting multiple genes including Mzb1. | ||
Arthritis Res Ther Increase of MZB1 in B cells in systemic lupus erythematosus: proteomic analysis of biopsied lymph nodes. | ||
Oncol Lett MZB1 expression indicates poor prognosis in estrogen receptor-positive breast cancer. |
