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  • KD/KO Validated

GKAP1 Polyclonal antibody

GKAP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11395-1-AP

Synonyms

FKSG21, G kinase anchoring protein 1, GKAP1, GKAP42



Tested Applications

Positive WB detected inHEK-293 cells, mouse testis tissue
Positive IHC detected inhuman cervical cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11395-1-AP targets GKAP1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GKAP1 fusion protein Ag1914
Full Name G kinase anchoring protein 1
Calculated Molecular Weight 366 aa, 42 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC014476
Gene Symbol GKAP1
Gene ID (NCBI) 80318
RRIDAB_2877761
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GKAP1, or GKAP42, is a novel male germ cell-specific 42-kDa protein isolated by Yuasa K et al. GKAP42 mRNA is specifically expressed in testis, where it is restricted to the spermatocytes and early round spermatids.

Protocols

Product Specific Protocols
WB protocol for GKAP1 antibody 11395-1-APDownload protocol
IHC protocol for GKAP1 antibody 11395-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB,IF,IHC

Mol Reprod Dev

Functional role of GKAP1 in the regulation of male germ cell spontaneous apoptosis and sperm number.

Authors - Hanshu Wang
  • KO Validated