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  • KD/KO Validated

ARMET Polyclonal antibody

ARMET Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10869-1-AP

Synonyms

Arginine rich protein, ARMET, ARP, MANF, Protein ARMET



Tested Applications

Positive WB detected inmouse pancreas tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10869-1-AP targets ARMET in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARMET fusion protein Ag1115
Full Name arginine-rich, mutated in early stage tumors
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC007282
Gene Symbol ARMET
Gene ID (NCBI) 7873
RRIDAB_2059300
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ARMET antibody 10869-1-APDownload protocol
IHC protocol for ARMET antibody 10869-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Commun

Imbalanced unfolded protein response signaling contributes to 1-deoxysphingolipid retinal toxicity

Authors - Jessica D Rosarda

J Am Soc Nephrol

Mesencephalic Astrocyte-Derived Neurotrophic Factor as a Urine Biomarker for Endoplasmic Reticulum Stress-Related Kidney Diseases.

Authors - Yeawon Kim
humanIHC

J Hepatocell Carcinoma

Identification and Validation of Novel Biomarkers for Hepatocellular Carcinoma, Liver Fibrosis/Cirrhosis and Chronic Hepatitis B via Transcriptome Sequencing Technology.

Authors - Dandan Zhao
humanWB

Eur J Oral Sci

Effect of mesencephalic astrocyte-derived neurotrophic factor on the inflammatory response in human gingival fibroblasts cells

Authors - Lei Gong
  • KD Validated
mouseWB

Int Immunopharmacol

Mesencephalic astrocyte-derived neurotrophic factor (MANF) alleviates cerebral ischemia/reperfusion injury in mice by regulating microglia polarization via A20/NF-κB pathway

Authors - Xueyan Zhao
humanWB

Front Oncol

DLC1 inhibits colon adenocarcinoma cell migration by promoting secretion of the neurotrophic factor MANF

Authors - Yi-Min Chu