Validation Data Gallery
Tested Applications
| Positive WB detected in | Daudi cells, mouse brain tissue, Raji cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 12 publications below |
| IF | See 6 publications below |
Product Information
10600-1-AP targets CD24 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0654 Product name: Recombinant human CD24 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-80 aa of BC007674 Sequence: MGRAMVARLGLGLLLLALLLPTQIYSSETTTGTSSNSSQSTSNSGLAPNPTNATTKAAGGALQSTASLFVVSLSLLHLYS 相同性解析による交差性が予測される生物種 |
| Full Name | CD24 molecule |
| Calculated molecular weight | 8 kDa |
| Observed molecular weight | 30-70 kDa |
| GenBank accession number | BC007674 |
| Gene Symbol | CD24 |
| Gene ID (NCBI) | 100133941 |
| RRID | AB_10646440 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P25063 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD24 (known as heat stable antigen) is a small highly glycosylated GPI-linked sialoprotein. It is normally expressed at the surface of most B lymphocytes and differentiating neuroblasts, and it is also up-regulated in a wide variety of cancers. Studies have shown that CD24 functions in the regulation of B-cell apoptosis, leukocyte signal transduction, and leukocyte adhesion. Since it is highly glycosylated, the apparent molecular weight of CD24 could be variable, ranging from 30 kDa to 70 kDa. (Ref: Akihiko Sano, MD., 2009)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD24 antibody 10600-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Microbiol Uropathogenic Escherichia coli invade luminal prostate cells via FimH-PPAP receptor binding. | ||
Nat Commun Natural photosynthetic system for restoring homeostasis of animal organelle interaction network. | ||
Exp Mol Med SLC38A4 promotes Kupffer cell phagocytosis and suppresses tumor liver metastasis. | ||
Adv Sci (Weinh) A Novel Long Non-Coding RNA lnc030 Maintains Breast Cancer Stem Cell Stemness by Stabilizing SQLE mRNA and Increasing Cholesterol Synthesis. | ||
Adv Sci (Weinh) Single‐Cell Transcriptome Profiling Reveals Multicellular Ecosystem of Nucleus Pulposus during Degeneration Progression | ||
Cancer Lett CD24 promotes metastasis and chemoresistance by directly targeting Arf6-ERK pathway in esophageal squamous cell carcinoma.
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