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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STARD5 Polyclonal antibody (10487-1-AP)

    WB analysis using 10487-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10487-1-AP (STARD5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using STARD5 Polyclonal antibody (10487-1-AP)

    WB analysis using 10487-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10487-1-AP (STARD5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using STARD5 Polyclonal antibody (10487-1-AP)

    IHC staining of human liver using 10487-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 10487-1-AP (STARD5 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using STARD5 Polyclonal antibody (10487-1-AP)

    IHC staining of mouse liver using 10487-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10487-1-AP (STARD5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using STARD5 Polyclonal antibody (10487-1-AP)

    IF Staining of HepG2 using 10487-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using STARD5 antibody (10487-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

STARD5 Polyclonal antibody

STARD5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 10487-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

StAR-related lipid transfer protein 5, START domain-containing protein 5

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCOLO 320 cells, HEK-293T cells, HepG2 cells, THP-1 cells
Positive IHC detected inmouse liver tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

10487-1-AP targets STARD5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0753

Product name: Recombinant human STARD5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-213 aa of BC004365

Sequence: MDPALAAQMSEAVAEKMLQYRRDTAGWKICREGNGVSVSWRPSVEFPGNLYRGEGIVYGTLEEVWDCVKPAVGGLRVKWDENVTGFEIIQSITDTLCVSRTSTPSAAMKLISPRDFVDLVLVKRYEDGTISSNATHVEHPLCPPKPGFVRGFNHPCGCFCEPLPGEPTKTNLVTFFHTDLSGYLPQNVVDSFFPRSMTRFYANLQKAVKQFHE

相同性解析による交差性が予測される生物種
Full Name StAR-related lipid transfer (START) domain containing 5
Calculated molecular weight 23.4 kDa
Observed molecular weight 24-26 kDa
GenBank accession numberBC004365
Gene Symbol STARD5
Gene ID (NCBI) 80765
RRIDAB_2197808
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NSY2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STARD5 (StAR-related lipid transfer domain containing 5) is a cytosolic lipid-transfer protein with a conserved START domain. It binds cholesterol and 25-hydroxycholesterol, maintaining intracellular sterol homeostasis. Its expression is induced under endoplasmic reticulum stress, triggering protein redistribution from nucleus to cytosol and membranes. STARD5 is highly expressed in liver, kidney and spleen; dysregulation links to hepatic steatosis, liver fibrosis and colorectal cancer progression by altering lipid metabolism. (PMID: 41954600)

Protocols

Product Specific Protocols
IF protocol for STARD5 antibody 10487-1-APDownload protocol
IHC protocol for STARD5 antibody 10487-1-APDownload protocol
WB protocol for STARD5 antibody 10487-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Oncol

AUP1 transcriptionally activated by KDM5B reprograms lipid metabolism to promote the malignant progression of cervical cancer

Authors - Yingping Zhu
View Article
mouseWB

PLoS One

Relationships between hematopoiesis and hepatogenesis in the midtrimester fetal liver characterized by dynamic transcriptomic and proteomic profiles.

Authors - Yuanbiao Guo
View Article
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