Mouse-IgG Polyclonal antibody

Mouse-IgG Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse and More (2)

Applications

WB, IP, RIP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 10283-1-AP

Synonyms

Mouse IgG


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  • Western Blot (WB) analysis of mouse serum using Mouse-IgG Polyclonal antibody (10283-1-AP)

    WB analysis of mouse serum using 10283-1-AP

    mouse serum were subjected to SDS PAGE followed by western blot with 10283-1-AP (Mouse-IgG antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

"Mouse-IgG Antibodies" Comparison

View side-by-side comparison of Mouse-IgG antibodies from other vendors to find the one that best suits your research needs.

Tested Applications

Positive WB detected inmouse serum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10283-1-AP targets Mouse-IgG in WB, IP, RIP, CoIP, ChIP, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant Protein
Full Name Mouse-IgG
Calculated Molecular Weight 155 kDa
Observed Molecular Weight 25 kDa, 50-55 kDa
Gene Symbol
Gene ID (NCBI)
RRIDAB_2877728
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody detectes all the heavy and light chains total mouse IgG.

Protocols

Product Specific Protocols
WB protocol for Mouse-IgG antibody 10283-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Nucleic Acids Res

An H3K4me3 reader, BAP18 as an adaptor of COMPASS-like core subunits co-activates ERα action and associates with the sensitivity of antiestrogen in breast cancer.

Authors - Ge Sun
humanWB

Comput Struct Biotechnol J

Impact of Cuproptosis-related markers on clinical status, tumor immune microenvironment and immunotherapy in colorectal cancer: A multi-omic analysis

Authors - Yanfei Shao

Cell Physiol Biochem

Long-Noncoding RNA Colorectal Neoplasia Differentially Expressed Gene as a Potential Target to Upregulate the Expression of IRX5 by miR-136-5P to Promote Oncogenic Properties in Hepatocellular Carcinoma.

Authors - Liying Zhu
IP

J Clin Endocrinol Metab

TRIM65 Promotes Invasion of Endometrial Stromal Cells by Activating ERK1/2/C-myc Signaling via Ubiquitination of DUSP6.

Authors - Ying-Ting Wu

Int J Mol Sci

Social Learning of Acquiring Novel Feeding Habit in Mandarin Fish (Siniperca chuatsi).

Authors - Jian Peng

Sci Rep

Premature termination codon readthrough in Drosophila varies in a developmental and tissue-specific manner.

Authors - Yanan Chen