Validation Data Gallery
-Antibody-14485-1-AP-WB-268098.jpg "WB analysis of MCF-7 using 14485-1-AP")
-Antibody-14485-1-AP-WB-428140.jpg "WB analysis of MCF-7 using 14485-1-AP")
-Antibody-14485-1-AP-WB-484367.jpg "WB analysis using 14485-1-AP")
-Antibody-14485-1-AP-WB-180462.jpg "WB analysis using 14485-1-AP")
-Antibody-14485-1-AP-IP-35809.jpg "IP experiment of HeLa using 14485-1-AP")
-Antibody-14485-1-AP-IHC-252307.jpg "IHC staining of human liver cancer using 14485-1-AP")
-Antibody-14485-1-AP-IF-17645.jpg "IF Staining of HepG2 using 14485-1-AP")
-Antibody-14485-1-AP-IF-408919.jpg "IF Staining of HepG2 using 14485-1-AP")
Tested Applications
| Positive WB detected in | HEK-293 cells, IGF-1 treated MCF-7 cells, Jurkat cells, NIH/3T3 cells, HeLa cells, PC-12 cells, C6 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 258 publications below |
| IHC | See 8 publications below |
| IF | See 6 publications below |
Product Information
14485-1-AP targets p70(S6K) in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, rabbit, chicken, bovine, megalobrama amblycephala |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5883 Product name: Recombinant human p70(S6K) protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC053365 Sequence: MRRRRRRDGFYPAPDFRDREAEDMAGVFDIDLDQPEDAGSEDELEEGGQLNESMDHGGVGPYELGMEHCEKFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVTGANTGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGENRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGPGDAGEVQAHPF 相同性解析による交差性が予測される生物種 |
| Full Name | ribosomal protein S6 kinase, 70kDa, polypeptide 1 |
| Calculated molecular weight | 59 kDa |
| Observed molecular weight | 65-75 kDa, 80-85 kDa |
| GenBank accession number | BC053365 |
| Gene Symbol | p70 S6K |
| Gene ID (NCBI) | 6198 |
| RRID | AB_2269787 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P23443 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RPS6KB1(Ribosomal protein S6 kinase beta-1) is also named as STK14A, p70 S6KA, and belongs to the S6 kinase subfamily. RPS6KB1 is a major substrate of mTOR and acts as a crucial effector of mTOR signaling pathway. It plays a key role in cell growth and proliferation by regulating INS sensitivity, metabolism, protein synthesis, and cell cycle. RPS6KB1 may play an important role in the progression of HCC and could serve as a potential molecular target for HCC therapy (PMID:22684641). The Rps6kb1 gene encodes the 70 kDa ribosomal protein S6 kinase (p70S6K). The PI3K/mTOR signalling pathway is one of the major mechanisms for controlling cell survival, proliferation, and metabolism and is the central regulator of translation of some components of the protein synthesis system. Due to alternative translation, two isoform S6K1 proteins are known to exist in mammalian cells: p85 S6K1 and p70 S6K1, which is identical to p85 S6K but lacks its first 23 amino acids. In addition, mammalian cells express a second S6K1 isoform spanning 316 amino acids (p31 S6K1).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for p70(S6K) antibody 14485-1-AP | Download protocol |
| IHC protocol for p70(S6K) antibody 14485-1-AP | Download protocol |
| IP protocol for p70(S6K) antibody 14485-1-AP | Download protocol |
| WB protocol for p70(S6K) antibody 14485-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther A CRISPR knockout negative screen reveals synergy between CDKs inhibitor and metformin in the treatment of human cancer in vitro and in vivo. | ||
Science Liver ALKBH5 regulates glucose and lipid homeostasis independently through GCGR and mTORC1 signaling | ||
Mol Cancer A novel polypeptide encoded by the circular RNA ZKSCAN1 suppresses HCC via degradation of mTOR | ||
Mol Cancer Role of the lncRNA ABHD11-AS1 in the tumorigenesis and progression of epithelial ovarian cancer through targeted regulation of RhoC. |