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  • Featured Product
  • KD/KO Validated

ZHX2 Monoclonal antibody

ZHX2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

4E12F2

Cat no : 68268-1-Ig

Synonyms

AFP regulator 1, AFR1, Alpha fetoprotein regulator 1, KIAA0854, RAF, Regulator of AFP, ZHX2, zinc fingers and homeoboxes 2

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 68268-1-Ig

    WB analysis of HEK-293 using 68268-1-Ig

    WB result of ZHX2 antibody (68268-1-Ig; 1:24000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZHX2 transfected HEK-293 cells.

  • WB analysis using 68268-1-Ig

    WB analysis using 68268-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68268-1-Ig (ZHX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

Tested Applications

Positive WB detected inSH-SY5Y cells, HEK-293 cells, LNCaP cells, HeLa cells, Jurkat cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy ZHX2 Ready-To-Use IHC Kit. ZHX2 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

68268-1-Ig targets ZHX2 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

Tested Reactivity Human, Mouse, Rat, Pig, Rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ZHX2 fusion protein Ag14420 相同性解析による交差性が予測される生物種
Full Name zinc fingers and homeoboxes 2
Calculated molecular weight 837 aa, 92 kDa
Observed molecular weight92-100 kDa
GenBank accession numberBC042145
Gene symbol ZHX2
Gene ID (NCBI) 22882
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Zinc fingers and homeoboxes protein 2 is a protein that in humans is encoded by the ZHX2 gene. ZHX2 Act as a transcriptional repressor, represses the promoter activity of the CDC25C gene stimulated by NFYA (PMID:12741956). In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family.

Protocols

Product Specific Protocols
WB protocol for ZHX2 antibody 68268-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols