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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZFP36L1 Monoclonal antibody

ZFP36L1 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1H6G6

Cat no : 68038-1-Ig

Synonyms

Berg36, BRF1, Butyrate response factor 1, cMG1, EGF response factor 1, ERF 1, ERF1, Protein TIS11B, RNF162B, TIS11B, ZFP36L1

Validation Data Gallery

Applications:
  • WB analysis using 68038-1-Ig

    WB analysis using 68038-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68038-1-Ig (ZFP36L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis of HSC-T6 using 68038-1-Ig

    WB analysis of HSC-T6 using 68038-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68038-1-Ig (ZFP36L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-12 using 68038-1-Ig

    WB analysis of PC-12 using 68038-1-Ig

    PC-12 cells were subjected to SDS PAGE followed by western blot with 68038-1-Ig (ZFP36L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 68038-1-Ig

    IF Staining of HepG2 using 68038-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ZFP36L1 antibody (68038-1-Ig, Clone: 1H6G6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHUVEC cells, HSC-T6 cells, PC-12 cells, U2OS cells, A549 cells, HeLa cells
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

68038-1-Ig targets ZFP36L1 in WB, IF, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ZFP36L1 fusion protein Ag30469 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 36, C3H type-like 1
Calculated molecular weight 338 aa, 36 kDa
Observed molecular weight36 kDa
GenBank accession numberBC018340
Gene symbol ZFP36L1
Gene ID (NCBI) 677
RRIDAB_2918780
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

As a member of the Tristetraprolin (TTP) RNA binding protein family, ZFP36L1 regulate gene expression post-transcriptionally by promoting mRNA decay. In mammals, ZFP36L1 and its family members have been shown to regulate development, cell diferentiation, cell cycle, apoptosis and anti-cancer capability by targeting an extensive overlapping repertoire of mRNAs.

Protocols

Product Specific Protocols
WB protocol for ZFP36L1 antibody 68038-1-IgDownload protocol
IF protocol for ZFP36L1 antibody 68038-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols