ZEB2 Monoclonal antibody

ZEB2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1D3A2

Cat no : 67514-1-Ig

Synonyms

FLJ42816, KIAA0569, SIP 1, SIP1, Smad interacting protein 1, SMADIP1, ZEB2, ZFHX1B, ZFX1B



Tested Applications

Positive WB detected inK-562 cells, HT-1080 cells, NIH/3T3 cells, HSC-T6 cells, 4T1 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

67514-1-Ig targets ZEB2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ZEB2 fusion protein Ag25477 相同性解析による交差性が予測される生物種
Full Name zinc finger E-box binding homeobox 2
Observed molecular weight 160 kDa
GenBank accession numberBC127102
Gene symbol ZEB2
Gene ID (NCBI) 9839
RRIDAB_2882735
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Zinc finger E-box-binding homeobox (ZEB2) is a key transcription factor that acts as a multifunctional regulator during nervous system development. ZEB2 contains two zinc finger domains and a homeodomain-like sequence and interacts with the TGF-ß superfamily signaling regulators, Smads, to regulate the expression of their downstream genes. ZEB2 is expressed in the developing neural tube, as well as in neural crest cells, the hippocampus and the cerebral cortex. ZEB2 has previously been implicated in EMT, cell-cycle progression, apoptosis and senescence. ZEB2 was overexpressed in bladder, ovarian, stomach, pancreatic and squamous cell carcinoma, in the intestinal subtype of stomach cancers, and at the invasive front of CRC where EMT is most prominen. ZEB2 also mediates cell-fate decision in neuronal, T cells and hematopoietic stem cells. The calculated molecular weight of ZEB2 is 136 kDa, but we find the 95 kDa band in some publication(PMID: 27659015 )

Protocols

Product Specific Protocols
WB protocol for ZEB2 antibody 67514-1-IgDownload protocol
IHC protocol for ZEB2 antibody 67514-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Med Chem

Discovery of a Novel Small-Molecule Inhibitor Disrupting TRBP-Dicer Interaction against Hepatocellular Carcinoma via the Modulation of microRNA Biogenesis.

Authors - Ting Peng
humanWB

Exp Cell Res

TRIM16 overexpression inhibits the metastasis of colorectal cancer through mediating Snail degradation.

Authors - Longhui Ruan
humanWB

Exp Physiol

ZEB1 recruit Brg1 to regulate airway remodeling epithelial-mesenchymal transition in asthma.

Authors - Ting Wang
humanWB,IF

Int J Med Sci

Lnc NR2F1-AS1 Promotes Breast Cancer Metastasis by Targeting the MiR-25-3p/ZEB2 Axis

Authors - Duanyang Zhai
  • KD Validated