Validation Data Gallery
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Tested Applications
| Positive WB detected in | K-562 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 15 publications below |
| IHC | See 2 publications below |
| IF | See 6 publications below |
| IP | See 4 publications below |
Product Information
25370-1-AP targets YOD1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, pig, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21881 Product name: Recombinant human YOD1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 63-348 aa of BC137167 Sequence: LSSRTRVRELQGQIAAITGIAPGGQRILVGYPPECLDLSNGDTILEDLPIQSGDMLIIEEDQTRPRSSPAFTKRGASSYVRETLPVLTRTVVPADNSCLFTSVYYVVEGGVLNPACAPEMRRLIAQIVASDPDFYSEAILGKTNQEYCDWIKRDDTWGGAIEISILSKFYQCEICVVDTQTVRIDRFGEDAGYTKRVLLIYDGIHYDPLQRNFPDPDTPPLTIFSSNDDIVLVQALELADEARRRRQFTDVNRFTLRCMVCQKGLTGQAEAREHAKETGHTNFGEV 相同性解析による交差性が予測される生物種 |
| Full Name | YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae) |
| Calculated molecular weight | 348 aa, 38 kDa |
| Observed molecular weight | 38 kDa |
| GenBank accession number | BC137167 |
| Gene Symbol | YOD1 |
| Gene ID (NCBI) | 55432 |
| RRID | AB_2880048 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5VVQ6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for YOD1 antibody 25370-1-AP | Download protocol |
| IP protocol for YOD1 antibody 25370-1-AP | Download protocol |
| WB protocol for YOD1 antibody 25370-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Pharm Sin B Blockade of deubiquitinase YOD1 degrades oncogenic PML/RARα and eradicates acute promyelocytic leukemia cells
| ||
Cell Death Dis Deubiquitinase YOD1 suppresses tumor progression by stabilizing E3 ligase TRIM33 in head and neck squamous cell carcinoma | ||
Cell Death Dis The deubiquitinase YOD1 suppresses tumor progression by stabilizing ZNF24 in clear cell renal carcinoma
| ||
Cell Death Discov miR-221/222 induce instability of p53 By downregulating deubiquitinase YOD1 in acute myeloid leukemia
| ||
Sci China Life Sci CRISPR screening identifies protein methylation and ubiquitination modifications that modulate aflatoxin B1 cytotoxicity |