Validation Data Gallery
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Tested Applications
| Positive WB detected in | K-562 cells, A375 cells, HEK-293 cells |
| Positive IP detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
20285-1-AP targets XRCC2 in WB, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14011 Product name: Recombinant human XRCC2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-280 aa of BC042137 Sequence: MCSAFHRAESGTELLARLEGRSSLKEIEPNLFADEDSPVHGDILEFHGPEGTGKTEMLYHLTARCILPKSEGGLEVEVLFIDTDYHFDMLRLVTILEHRLSQSSEEIIKYCLGRFFLVYCSSSTHLLLTLYSLESMFCSHPSLCLLILDSLSAFYWIDRVNGGESVNLQESTLRKCSQCLEKLVNDYRLVLFATTQTIMQKASSSSEEPSHASRRLCDVDIDYRPYLCKAWQQLVKHRMFFSKQDDSQSSNQFSLVSRCLKSNSLKKHFFIIGESGVEFC 相同性解析による交差性が予測される生物種 |
| Full Name | X-ray repair complementing defective repair in Chinese hamster cells 2 |
| Calculated molecular weight | 280 aa, 32 kDa |
| Observed molecular weight | 35-40 kDa |
| GenBank accession number | BC042137 |
| Gene Symbol | XRCC2 |
| Gene ID (NCBI) | 7516 |
| RRID | AB_2878662 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43543 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for XRCC2 antibody 20285-1-AP | Download protocol |
| WB protocol for XRCC2 antibody 20285-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Struct Mol Biol Fanconi anemia DNA crosslink repair factors protect against LINE-1 retrotransposition during mouse development | ||
PLoS One The dysregulation of PARP9 expression is linked to apoptosis and DNA damage in gastric cancer cells |
