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KD/KO Validated

XRCC1 Polyclonal antibody

Name: XRCC1 Polyclonal antibody
Brand: Proteintech
SKU: 21468-1-AP

XRCC1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 21468-1-AP

Synonyms

DNA repair protein XRCC1, RCC, XRCC1

Validation Data Gallery

Applications:

AllWBIF

WB analysis using 21468-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21468-1-AP (XRCC1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 21468-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 21468-1-AP (XRCC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 21468-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 21468-1-AP (XRCC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 21468-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 21468-1-AP (XRCC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 21468-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21468-1-AP (XRCC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21468-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21468-1-AP (XRCC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 21468-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 21468-1-AP (XRCC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 21468-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 21468-1-AP (XRCC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IF Staining of HeLa using 21468-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 21468-1-AP (XRCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 21468-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, Jurkat cells, K-562 cells, HeLa cells
Positive IF detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunofluorescence (IF)	IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 2 publications below
WB	See 10 publications below
IHC	See 1 publications below

Product Information

The immunogen of 21468-1-AP is XRCC1 Fusion Protein expressed in E. coli.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	XRCC1 fusion protein Ag15172 相同性解析による交差性が予測される生物種
Full Name	X-ray repair complementing defective repair in Chinese hamster cells 1
Calculated molecular weight	633 aa, 70 kDa
Observed molecular weight	80-90 kDa
GenBank accession number	BC023593
Gene symbol	XRCC1
Gene ID (NCBI)	7515
RRID	AB_2878865
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for XRCC1 antibody 21468-1-AP	Download protocol
IF protocol for XRCC1 antibody 21468-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nucleic Acids Res KDM6B promotes PARthanatos via suppression of O6-methylguanine DNA methyltransferase repair and sustained checkpoint response. Authors - Mingming Yang View Article
human	WB	Mol Ther PMID: 29456019 Authors - Xinyi Wang View Article
human	WB	J Cell Mol Med Berberine attenuates XRCC1-mediated base excision repair and sensitizes breast cancer cells to the chemotherapeutic drugs. Authors - Xingjie Gao View Article
human	WB	Int J Environ Res Public Health Effects of DNA Damage and Oxidative Stress in Human Bronchial Epithelial Cells Exposed to PM2.5 from Beijing, China, in Winter. Authors - Bing-Yu Niu View Article
human	WB	Toxicol Appl Pharmacol Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis. Authors - Hui Dong View Article
human	WB,IHC	Front Mol Biosci XRCC1 Is a Promising Predictive Biomarker and Facilitates Chemo-Resistance in Gallbladder Cancer. Authors - Zhengchun Wu KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

XRCC1 Polyclonal antibody

XRCC1 Polyclonal Antibody for WB, IF, ELISA

Validation Data Gallery

WB analysis using 21468-1-AP

WB analysis of K-562 using 21468-1-AP

WB analysis of Jurkat using 21468-1-AP

WB analysis of Jurkat using 21468-1-AP

WB analysis of HEK-293 using 21468-1-AP

WB analysis of HeLa using 21468-1-AP

WB analysis of K-562 using 21468-1-AP

WB analysis of Jurkat using 21468-1-AP

IF Staining of HeLa using 21468-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

KDM6B promotes PARthanatos via suppression of O6-methylguanine DNA methyltransferase repair and sustained checkpoint response.

PMID: 29456019

Berberine attenuates XRCC1-mediated base excision repair and sensitizes breast cancer cells to the chemotherapeutic drugs.

Effects of DNA Damage and Oxidative Stress in Human Bronchial Epithelial Cells Exposed to PM2.5 from Beijing, China, in Winter.

Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis.

XRCC1 Is a Promising Predictive Biomarker and Facilitates Chemo-Resistance in Gallbladder Cancer.