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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

WWP1 Monoclonal antibody

WWP1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G8G11

Cat no : 67804-1-Ig

Synonyms

AIP5, DKFZp434D2111, hSDRP1, Tiul1, WW domain containing protein 1, WWP1

Validation Data Gallery

Applications:
  • WB analysis using 67804-1-Ig

    WB analysis using 67804-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67804-1-Ig (WWP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • WB analysis of HaCaT using 67804-1-Ig

    WB analysis of HaCaT using 67804-1-Ig

    HaCaT cells were subjected to SDS PAGE followed by western blot with 67804-1-Ig (WWP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inMCF-7 cells, HaCaT cells, HeLa cells, LNCaP cells, Jurkat cells
Planning an IHC experiment? We recommend our IHCeasy WWP1 Ready-To-Use IHC Kit. WWP1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

67804-1-Ig targets WWP1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen WWP1 fusion protein Ag30171 相同性解析による交差性が予測される生物種
Full Name WW domain containing E3 ubiquitin protein ligase 1
Calculated molecular weight 922 aa, 105 kDa
Observed molecular weight 95-105 kDa
GenBank accession numberBC036065
Gene symbol WWP1
Gene ID (NCBI) 11059
RRIDAB_2918567
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WW domain-containing E3 ubiquitin protein ligase 1 (WWP1) is a multifunction protein containing an N-terminal C2 domain, four tandem WW domains for substrate binding, and a C-terminal catalytic HECT domain for ubiquitin transferring. It is also named as AIP5, Tiul1. WWP1 regulates a variety of cellular biological processes including protein trafficking and degradation, signaling, transcription, and viral budding. WWP1 has been implicated in several diseases, such as cancers, infectious diseases, neurological diseases, and aging(PMID:22051607). It has 6 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for WWP1 antibody 67804-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols