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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

WWP1 Polyclonal antibody

WWP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28689-1-AP

Synonyms

AIP5, DKFZp434D2111, hSDRP1, Tiul1, WW domain containing protein 1, WWP1

Validation Data Gallery

Applications:
  • WB analysis of HepG2 using 28689-1-AP

    WB analysis of HepG2 using 28689-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 28689-1-AP (WWP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 28689-1-AP

    WB analysis of HeLa using 28689-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 28689-1-AP (WWP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 28689-1-AP

    WB analysis of MCF-7 using 28689-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 28689-1-AP (WWP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 28689-1-AP

    WB analysis of A431 using 28689-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 28689-1-AP (WWP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 28689-1-AP

    IHC staining of human breast cancer using 28689-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28689-1-AP (WWP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with WWP1 antibody included
  • IHC staining of human breast cancer using 28689-1-AP

    IHC staining of human breast cancer using 28689-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28689-1-AP (WWP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with WWP1 antibody included
  • IHC staining of human liver cancer using 28689-1-AP

    IHC staining of human liver cancer using 28689-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28689-1-AP (WWP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with WWP1 antibody included
  • IHC staining of human liver cancer using 28689-1-AP

    IHC staining of human liver cancer using 28689-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28689-1-AP (WWP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with WWP1 antibody included

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, MCF-7 cells, A431 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy WWP1 Ready-To-Use IHC Kit. WWP1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

28689-1-AP targets WWP1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WWP1 fusion protein Ag30161 相同性解析による交差性が予測される生物種
Full Name WW domain containing E3 ubiquitin protein ligase 1
Calculated molecular weight 922 aa, 105 kDa
Observed molecular weight 95-105 kDa
GenBank accession numberBC036065
Gene symbol WWP1
Gene ID (NCBI) 11059
RRIDAB_2881195
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WW domain-containing E3 ubiquitin protein ligase 1 (WWP1) is a multifunction protein containing an N-terminal C2 domain, four tandem WW domains for substrate binding, and a C-terminal catalytic HECT domain for ubiquitin transferring. It is also named as AIP5, Tiul1. WWP1 regulates a variety of cellular biological processes including protein trafficking and degradation, signaling, transcription, and viral budding. WWP1 has been implicated in several diseases, such as cancers, infectious diseases, neurological diseases, and aging(PMID:22051607). It has 6 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for WWP1 antibody 28689-1-APDownload protocol
IHC protocol for WWP1 antibody 28689-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Med

Quercetin-induced degradation of RhoC suppresses hepatocellular carcinoma invasion and metastasis

Authors - Chunlong Huang
View Article