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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

WNK1 Monoclonal antibody

WNK1 Monoclonal Antibody for WB, FC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1B6A10

Cat no : 68149-1-Ig

Synonyms

Erythrocyte 65 kDa protein, HSAN2, HSN2, hWNK1, KDP, KIAA0344, Kinase deficient protein, p65, PHA2C, PRKWNK1, PSK, WNK1

Validation Data Gallery

Applications:
  • WB analysis using 68149-1-Ig

    WB analysis using 68149-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68149-1-Ig (WNK1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • FC experiment of HEK-293T using 68149-1-Ig

    FC experiment of HEK-293T using 68149-1-Ig

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human WNK1 (68149-1-Ig, Clone:1B6A10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, A549 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive FC detected inHEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy WNK1 Ready-To-Use IHC Kit. WNK1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

68149-1-Ig targets WNK1 in WB, FC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen WNK1 fusion protein Ag28044 相同性解析による交差性が予測される生物種
Full Name WNK lysine deficient protein kinase 1
Calculated molecular weight 251 kDa
Observed molecular weight250 kDa
GenBank accession numberBC021121
Gene symbol WNK1
Gene ID (NCBI) 65125
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WNK kinase is a unique serine-threonine kinase in which the lysine (K) residue in the catalytic domain, that is found in the majority of kinases is not conserved. In mammals, there are four WNK kinase family members (WNK1-4). WNK1 has 6 isoforms, such as long form WNK1 (L-WNK1, 250 kDa, strong expression in dorsal root ganglia and spinal cord) ; kidney-specific isoform (KS-WNK1, 207 kDa, lacks a kinase domain).KS-WNK1 cannot phosphorylate substrates and instead interacts with and modulates the actions of other WNKs via a carboxyl terminal HQ domain.

Protocols

Product Specific Protocols
WB protocol for WNK1 antibody 68149-1-IgDownload protocol
FC protocol for WNK1 antibody 68149-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols