• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

WDR18 Polyclonal antibody

WDR18 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15165-1-AP

Synonyms

R32184_1, WD repeat domain 18, WDR18

Validation Data Gallery

Applications:
  • WB analysis of Jurkat using 15165-1-AP

    WB analysis of Jurkat using 15165-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15165-1-AP (WDR18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 15165-1-AP

    WB analysis of Jurkat using 15165-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15165-1-AP (WDR18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung using 15165-1-AP

    IHC staining of human lung using 15165-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15165-1-AP (WDR18 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung using 15165-1-AP

    IHC staining of human lung using 15165-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15165-1-AP (WDR18 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 15165-1-AP

    IHC staining of human ovary tumor using 15165-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15165-1-AP (WDR18 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human ovary tumor using 15165-1-AP

    IHC staining of human ovary tumor using 15165-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15165-1-AP (WDR18 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human lung cancer using 15165-1-AP

    IHC staining of human lung cancer using 15165-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 15165-1-AP (WDR18 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lung cancer using 15165-1-AP

    IHC staining of human lung cancer using 15165-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 15165-1-AP (WDR18 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman lung tissue, human lung cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15165-1-AP targets WDR18 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WDR18 fusion protein Ag7282 相同性解析による交差性が予測される生物種
Full Name WD repeat domain 18
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC001648
Gene symbol WDR18
Gene ID (NCBI) 57418
RRIDAB_2288469
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WDR18 is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. WDR18 may play a role during development. The complex PELP1-TEX10-WDR18 is required for the maturation and nucleolar release of the large ribosomal subunit. This complex is a regulator of ribosome biogenesis and suggests that its SUMO-controlled distribution coordinates the rate of ribosome formation (PMID:21326211).

Protocols

Product Specific Protocols
WB protocol for WDR18 antibody 15165-1-APDownload protocol
IHC protocol for WDR18 antibody 15165-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO J

The SUMO system controls nucleolar partitioning of a novel mammalian ribosome biogenesis complex.

Authors - Finkbeiner Elisabeth E
  • KD Validated
View Article
humanWB

Mol Cell Proteomics

Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation.

Authors - Fanis Pavlos P
View Article
humanWB

PLoS One

A Role of hIPI3 in DNA Replication Licensing in Human Cells.

Authors - Yining Huang
View Article
humanIP,WB

Mol Oncol

PELP1 inhibition by SMIP34 reduces endometrial cancer progression via attenuation of ribosomal biogenesis

Authors - Xue Yang
View Article