Validation Data Gallery
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Tested Applications
| Positive IHC detected in | rat brain tissue, mouse brain tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 3 publications below |
| IF | See 2 publications below |
| FC | See 1 publications below |
Product Information
55009-1-AP targets WDFY3/ALFY in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | WD repeat and FYVE domain containing 3 |
| Calculated molecular weight | 395 kDa |
| GenBank accession number | NM_014991 |
| Gene Symbol | WDFY3 |
| Gene ID (NCBI) | 23001 |
| RRID | AB_10863126 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IZQ1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
WDFY3, also named as KIAA0993 and Alfy, is a protein which contains WD repeats and an FYVE domain. Multiple alternatively spliced transcript variants have been found for WDFY3, but the full-length nature of some variants has not been defined. The antibody is specific to WDFY3.The band 50kd in WB is a splicing form of WDFY3.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for WDFY3/ALFY antibody 55009-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cardiovasc Res WD40 repeat and FYVE domain containing 3 (Wdfy3) is essential for cardiac development.
| ||
In Vitro Cell Dev Biol Anim Autophagy-linked FYVE protein (Alfy) promotes autophagic removal of misfolded proteins involved in amyotrophic lateral sclerosis (ALS). | ||
Res Sq Genetic depletion of early autophagy protein ATG13 impairs mitochondrial energy metabolism, augments oxidative stress, induces the polarization of macrophages to M1 inflammatory mode, and compromises myelin integrity in skeletal muscle. | ||
Mol Cell Proteomics Spinal cord phosphoproteome of SCA2 mouse model reveals alteration of ATXN2-N-term PRM-SH3-actin interactome and of autophagy |