Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 10987-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10987-1-AP (WASP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 10987-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 10987-1-AP (WASP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 10987-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10987-1-AP (WASP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 10987-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 10987-1-AP (WASP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 10987-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 10987-1-AP (WASP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of Raji using 10987-1-AP
IP result of anti-WASP (IP:10987-1-AP, 4ug; Detection:10987-1-AP 1:5000) with Raji cells lysate 1600 ug.
IP experiment of rat spleen using 10987-1-AP
IP result of anti-WASP (IP:10987-1-AP, 4ug; Detection:10987-1-AP 1:500) with rat spleen tissue lysate 4000 ug.
IHC staining of human spleen using 10987-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 10987-1-AP (WASP antibody at dilution of 1:50 (under 40x lens).
IHC staining of human tonsillitis using 10987-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10987-1-AP (WASP antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 10987-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10987-1-AP (WASP antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 10987-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10987-1-AP (WASP antibody at dilution of 1:50 (under 10x lens).
IHC staining of human tonsillitis using 10987-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10987-1-AP (WASP antibody at dilution of 1:50 (under 40x lens).
IHC staining of human lymphoma using 10987-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10987-1-AP (WASP antibody at dilution of 1:50 (under 10x lens).
IHC staining of human lymphoma using 10987-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10987-1-AP (WASP antibody at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO Validated

WASP Polyclonal antibody

WASP Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 10987-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, RIP, ELISA

Synonyms

Wiskott-Aldrich syndrome protein, WAS, Actin nucleation-promoting factor WAS

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (5)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HL-60 cells, K-562 cells, mouse spleen tissue, Jurkat cells, Raji cells
Positive IP detected in	Raji cells, rat spleen tissue
Positive IHC detected in	human tonsillitis tissue, human lymphoma tissue, human spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IP	See 1 publications below
RIP	See 1 publications below

Product Information

10987-1-AP targets WASP in WB, IHC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	WASP fusion protein Ag1444 相同性解析による交差性が予測される生物種
Full Name	Wiskott-Aldrich syndrome (eczema-thrombocytopenia)
Calculated molecular weight	53 kDa
Observed molecular weight	55-62 kDa
GenBank accession number	BC012738
Gene Symbol	WASP
Gene ID (NCBI)	7454
RRID	AB_2304546
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P42768
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Wiskott-Aldrich Syndrome protein (WASP) regulates actin cytoskeleton in hematopoietic cells and defects in WASP cause Wiskott-Aldrich Syndrome (WAS), an X-linked immunodeficiency and autoimmunity disorder of childhood.

Protocols

Product Specific Protocols
WB protocol for WASP antibody 10987-1-AP	Download protocol
IHC protocol for WASP antibody 10987-1-AP	Download protocol
IP protocol for WASP antibody 10987-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	J Allergy Clin Immunol Dedicator of cytokinesis protein 2 couples with lymphoid enhancer-binding factor 1 to regulate expression of CD21 and B-cell differentiation. Authors - Yukai Jing View Article
human		Nat Commun WASp modulates RPA function on single-stranded DNA in response to replication stress and DNA damage. Authors - Seong-Su Han View Article
human	WB	J Exp Clin Cancer Res miR-182 suppresses invadopodia formation and metastasis in non-small cell lung cancer by targeting cortactin gene. Authors - Yongwen Li KD Validated View Article
human	WB	J Exp Clin Cancer Res CDC42-interacting protein 4 promotes metastasis of nasopharyngeal carcinoma by mediating invadopodia formation and activating EGFR signaling. Authors - Dong-Fang Meng View Article
human	WB,RIP,IP	Mol Cancer Res LINC00869 Promotes Hepatocellular Carcinoma Metastasis via Protrusion Formation Authors - Xiaowen Shao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10987-1-AP

WB analysis of Raji using 10987-1-AP

WB analysis of mouse spleen using 10987-1-AP

WB analysis of HL-60 using 10987-1-AP

WB analysis of K-562 using 10987-1-AP

IP experiment of Raji using 10987-1-AP

IP experiment of rat spleen using 10987-1-AP

IHC staining of human spleen using 10987-1-AP

IHC staining of human tonsillitis using 10987-1-AP

IHC staining of human tonsillitis using 10987-1-AP

IHC staining of human tonsillitis using 10987-1-AP

IHC staining of human tonsillitis using 10987-1-AP

IHC staining of human lymphoma using 10987-1-AP

IHC staining of human lymphoma using 10987-1-AP

WASP Polyclonal antibody

WASP Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Dedicator of cytokinesis protein 2 couples with lymphoid enhancer-binding factor 1 to regulate expression of CD21 and B-cell differentiation.

WASp modulates RPA function on single-stranded DNA in response to replication stress and DNA damage.

miR-182 suppresses invadopodia formation and metastasis in non-small cell lung cancer by targeting cortactin gene.

CDC42-interacting protein 4 promotes metastasis of nasopharyngeal carcinoma by mediating invadopodia formation and activating EGFR signaling.

LINC00869 Promotes Hepatocellular Carcinoma Metastasis via Protrusion Formation