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Vimentin Monoclonal antibody
Vimentin Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, FC, CoIP, ELISA
Conjugate
Unconjugated
201
CloneNo.
3H9D1
Cat no : 60330-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, NIH/3T3 cells, ROS1728 cells, Jurkat cells, A549 cells, U2OS cells, U-251 cells |
| Positive IHC detected in | human appendicitis tissue, human renal cell carcinoma tissue, human endometrial cancer tissue, human ovary tumor tissue, human breast cancer tissue, human colon tissue, human liver cancer tissue, human tonsillitis tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HUVEC cells, Retinal organoids, HepG2 cells |
| Positive FC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
| Immunofluorescence (IF) | IF : 1:500-1:2000 |
| Flow Cytometry (FC) | FC : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Product Information
60330-1-Ig targets Vimentin in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, mouse, zebrafish |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Vimentin fusion protein Ag0489 相同性解析による交差性が予測される生物種 |
| Full Name | vimentin |
| Calculated molecular weight | 466 aa, 54 kDa |
| Observed molecular weight | 55-60 kDa |
| GenBank accession number | BC000163 |
| Gene symbol | VIM |
| Gene ID (NCBI) | 7431 |
| RRID | AB_2881439 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Vimentin antibody 60330-1-Ig | Download protocol |
| IHC protocol for Vimentin antibody 60330-1-Ig | Download protocol |
| IF protocol for Vimentin antibody 60330-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Mater Neonatal Tissue-derived Extracellular Vesicle Therapy (NEXT): A Potent Strategy for Precision Regenerative Medicine | ||
Mol Cancer CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer. | ||
Acta Pharm Sin B Celastrol induces ferroptosis in activated HSCs to ameliorate hepatic fibrosis via targeting peroxiredoxins and HO-1. | ||
J Pineal Res Melatonin and verteporfin synergistically suppress the growth and stemness of head and neck squamous cell carcinoma through the regulation of mitochondrial dynamics | ||
Aging Cell Müller Glia maintain their regenerative potential despite degeneration in the aged zebrafish retina. | ||
Aging Cell Lysyl oxidase-like 2 inhibitor rescues D-galactose-induced skeletal muscle fibrosis. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Alessandro (Verified Customer) (12-09-2023) | good IF antibody. no unspecific staining
|
FH Aarushi (Verified Customer) (04-15-2023) | Primary antibody worked well after methanol treatment in a dilution of 1:200.
 |
FH Saba (Verified Customer) (03-03-2022) | The antibody worked perfectly!! The band was sharp and no unspecefic bands were seen.
|
