Validation Data Gallery

Filter: AllWBIPIF/ICC

WB analysis of human brain using 15079-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 15079-1-AP (VPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 15079-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15079-1-AP (VPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 15079-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 15079-1-AP (VPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 15079-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 15079-1-AP (VPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 15079-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15079-1-AP (VPS8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 15079-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15079-1-AP (VPS8 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
IP experiment of mouse brain using 15079-1-AP
IP result of anti-VPS8 (IP:15079-1-AP, 4ug; Detection:15079-1-AP 1:600) with mouse brain tissue lysate 4000ug.
IF Staining of HepG2 using 15079-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 15079-1-AP (VPS8 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

VPS8 Polyclonal antibody

VPS8 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 15079-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

KIAA0804, Vacuolar protein sorting-associated protein 8 homolog

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human brain tissue, human testis tissue, mouse brain tissue, mouse liver tissue, PC-3 cells
Positive IP detected in	mouse brain tissue
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2400
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 4 publications below
IP	See 1 publications below

Product Information

15079-1-AP targets VPS8 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7151 Product name: Recombinant human VPS8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 56-147 aa of BC001001 Sequence: MSPSYHQSKGDPTAKKGTSEPVLDPQQIQAFDQLCRLYRGSSRLALLTELSQNRSSESYRPFSGSQSAPAFNSIFQNENFQLQLIPPPVTED 相同性解析による交差性が予測される生物種
Full Name	vacuolar protein sorting 8 homolog (S. cerevisiae)
Calculated molecular weight	162 kDa
Observed molecular weight	151 kDa
GenBank accession number	BC001001
Gene Symbol	VPS8
Gene ID (NCBI)	23355
RRID	AB_2215543
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8N3P4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

VPS8, also named as KIAA0804, has some isoforms with MW 135-160 kDa and 70-90 kDa.

Protocols

Product Specific Protocols
IF protocol for VPS8 antibody 15079-1-AP	Download protocol
IP protocol for VPS8 antibody 15079-1-AP	Download protocol
WB protocol for VPS8 antibody 15079-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Elife Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors. Authors - Dawei Wang KD Validated View Article
human	WB,IP	Traffic Mammalian CORVET is required for fusion and conversion of distinct early endosome sub-populations. Authors - Enrico Daniele Perini View Article
human	WB	Biochem J VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction. Authors - Morag R Hunter View Article
human	WB	Sci Rep HOPS, CORVET and newly-identified Hybrid tethering complexes contribute differentially towards multiple modes of endocytosis Authors - Seigo Terawaki KO Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human brain using 15079-1-AP

WB analysis of mouse liver using 15079-1-AP

WB analysis of human testis using 15079-1-AP

WB analysis of PC-3 using 15079-1-AP

WB analysis of mouse brain using 15079-1-AP

WB analysis of mouse brain using 15079-1-AP

IP experiment of mouse brain using 15079-1-AP

IF Staining of HepG2 using 15079-1-AP