Validation Data Gallery

Filter: AllWBIPIF/ICC

WB analysis of human brain using 15079-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 15079-1-AP (VPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 15079-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15079-1-AP (VPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 15079-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 15079-1-AP (VPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 15079-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 15079-1-AP (VPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 15079-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15079-1-AP (VPS8 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
WB analysis of mouse brain using 15079-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15079-1-AP (VPS8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 15079-1-AP
IP result of anti-VPS8 (IP:15079-1-AP, 4ug; Detection:15079-1-AP 1:600) with mouse brain tissue lysate 4000ug.
IF Staining of HepG2 using 15079-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 15079-1-AP (VPS8 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

VPS8 Polyclonal antibody

VPS8 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 15079-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

Vacuolar protein sorting-associated protein 8 homolog, KIAA0804

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human brain tissue, human testis tissue, mouse brain tissue, mouse liver tissue, PC-3 cells
Positive IP detected in	mouse brain tissue
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2400
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 4 publications below
IP	See 1 publications below

Product Information

15079-1-AP targets VPS8 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	VPS8 fusion protein Ag7151 相同性解析による交差性が予測される生物種
Full Name	vacuolar protein sorting 8 homolog (S. cerevisiae)
Calculated molecular weight	162 kDa
Observed molecular weight	151 kDa
GenBank accession number	BC001001
Gene Symbol	VPS8
Gene ID (NCBI)	23355
RRID	AB_2215543
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8N3P4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

VPS8, also named as KIAA0804, has some isoforms with MW 135-160 kDa and 70-90 kDa.

Protocols

Product Specific Protocols
WB protocol for VPS8 antibody 15079-1-AP	Download protocol
IF protocol for VPS8 antibody 15079-1-AP	Download protocol
IP protocol for VPS8 antibody 15079-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Elife Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors. Authors - Dawei Wang KD Validated View Article
human	WB, IP	Traffic Mammalian CORVET is required for fusion and conversion of distinct early endosome sub-populations. Authors - Enrico Daniele Perini View Article
human	WB	Biochem J VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction. Authors - Morag R Hunter View Article
human	WB	Sci Rep HOPS, CORVET and newly-identified Hybrid tethering complexes contribute differentially towards multiple modes of endocytosis Authors - Seigo Terawaki KO Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human brain using 15079-1-AP

WB analysis of mouse liver using 15079-1-AP

WB analysis of human testis using 15079-1-AP

WB analysis of PC-3 using 15079-1-AP

WB analysis of mouse brain using 15079-1-AP

WB analysis of mouse brain using 15079-1-AP

IP experiment of mouse brain using 15079-1-AP

IF Staining of HepG2 using 15079-1-AP